Plasma protein adsorption pattern on characterized ceramic biomaterials
Plasma protein adsorption pattern on characterized ceramic biomaterials
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DOI：
10.1016/s0142-9612(01)00244-7
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发表时间：
2002-02-01
期刊：
影响因子：
14
通讯作者：
Piancastelli, A
中科院分区：
文献类型：
--
作者：
Rosengren, Å;Pavlovic, E;Piancastelli, A
对三种硬组织外科用生物材料的蛋白质/生物材料相互作用进行了体外研究。采用动态流动系统和二维聚丙烯酰胺凝胶电泳（2D-PAGE）研究了羟基磷灰石、氧化铝和氧化锆对稀释人血浆蛋白的吸附，包括总蛋白结合量、特异性蛋白的相对结合量以及流动和解吸模式。陶瓷的特点是关于物理化学性质，即，化学成分的元素分析和比表面积，孔体积和孔径分布使用BET法和Hg-porosimetry.The材料被发现吸附了令人惊讶的低量的血浆蛋白质，离开超过70%的表面免费。因此，与完全覆盖蛋白质的表面相反，细胞反应将受到材料物理化学性质的高度影响。关于蛋白质的吸附，大多数蛋白质在三种吸附剂上表现出相似的流过模式。载脂蛋白D（ApoD）、载脂蛋白J（ApoJ）、补体因子C1 s（C1 s）除外。补体因子C3（C3）、血浆铜蓝蛋白、纤维蛋白原、α（1）B糖蛋白和α（2）HS糖蛋白以及血清视网膜结合蛋白（SRBP）。这些蛋白质在接受或排斥植入物的作用必须进行研究。(C)2001爱思唯尔科技有限公司版权所有。
The protein/biomaterial interactions of three biomaterials used in hard tissue surgery were studied in vitro. A dynamic flow system and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were used to investigate the adsorption of proteins from diluted human plasma on hydroxyapatite, alumina and zirconia, with regard to total protein binding capacity, relative binding capacity for specific proteins and flow-through and desorption patterns. The ceramics were characterized regarding physicochemical properties, namely, chemical composition by elementary analyses and specific surface, pore volume and pore size distribution using the BET-method and Hg-porosimetry.The materials were found to adsorb a surprisingly low amount of plasma proteins, leaving more than 70% of the surface free. The cellular response will therefore be highly affected by the physico-chemical properties of the material, in contrast to a surface fully covered with proteins. Regarding the adsorption of proteins, most proteins exhibited similar flow-through patterns on the three adsorbents. The exceptions with different flow-through patterns were apolipoprotein D (Apo D), apolipoprotein J (Apo J), complement factor C1s (C1s). complement factor C3 (C3), ceruloplasmin, fibrinogen, alpha (1) B glycoprotein and alpha (2) HS glycoprotein a nd serum retinal-binding protein (SRBP). The role of these proteins on acceptance or rejection of implants has to be investigated. (C) 2001 Elsevier Science Ltd. All rights reserved.