Evaluation of permselective membranes for optimization of intracerebral amperometric glutamate biosensors.
Evaluation of permselective membranes for optimization of intracerebral amperometric glutamate biosensors.
复制标题
DOI：
10.1016/j.bios.2012.01.019
复制
发表时间：
2012-03
影响因子：
12.6
通讯作者：
N. Wahono;S. Qin;P. Oomen;T. Cremers;M. D. de Vries;B. Westerink
中科院分区：
文献类型：
--
作者：
N. Wahono;S. Qin;P. Oomen;T. Cremers;M. D. de Vries;B. Westerink
通过脑内生物传感器监测细胞外脑谷氨酸浓度是进一步研究这一重要神经递质作用的一种有希望的方法。然而，安培生物传感器通常受到大脑中其他电活性物质（如抗坏血酸、多巴胺和尿酸）引起的法拉第干扰的阻碍。为了防止这种情况发生，生物传感器上经常使用各种过选择性膜。在这项研究中，我们评估了最常用的膜，即钠离子，聚苯二胺，聚吡咯，聚苯胺和聚萘酚使用一种新的硅基铂电极。首先，我们选择了对过氧化氢具有最高敏感性和对电化学干扰具有最佳选择性的膜。然后，我们在短时间（3-4h）的体内实验中评估了这些膜的性能。我们发现，通过循环伏安法沉积在铂电极上的聚（间苯二胺）膜保护的生物传感器具有最佳的体外性能。然而，植入后的评价表明这些膜对多巴胺的选择性较差。与先前应用的国民层结合不能保护传感器免受急性生物污染；事实上，这甚至起到了反作用。最后，我们研究了生物传感器监测谷氨酸转运阻滞剂DL-TBOA对麻醉大鼠前额皮质谷氨酸浓度调节的作用。优化后的生物传感器记录了细胞外谷氨酸快速增加35倍，并且被认为适合进一步在体内进行探索
Monitoring of extracellular brain glutamate concentrations by intracerebral biosensors is a promising approach to further investigate the role of this important neurotransmitter. However, amperometric biosensors are typically hampered by Faradaic interference caused by the presence of other electroactive species in the brain, such as ascorbic acid, dopamine, and uric acid. Various permselective membranes are often used on biosensors to prevent this. In this study we evaluated the most commonly used membranes, i.e. nafion, polyphenylenediamine, polypyrrole, polyaniline, and polynaphthol using a novel silica-based platinum electrode. First we selected the membranes with the highest sensitivity for hydrogen peroxide in vitro and an optimal selectivity against electrochemical interferents. Then we evaluated the performances of these membranes in a short lasting (3–4h) in vivo experiment. We found that best in vitro performance was accomplished with biosensors that were protected by a poly(m-phenylenediamine) membrane deposited onto the platinum electrode by cyclic voltammetry. However, post-implantation evaluation of these membranes showed poor selectivity against dopamine. Combination with a previously applied nafion layer did not protect the sensors against acute biofouling; indeed it was even counter effective. Finally, we investigated the ability of our biosensors to monitor the effect of glutamate transport blocker DL-TBOA on modulating glutamate concentrations in the prefrontal cortex of anaesthetized rats. The optimized biosensors recorded a rapid 35-fold increase in extracellular glutamate, and are considered suitable for further exploration in vivo