Steroidogenic fate of the Leydig cells that repopulate the testes of young and aged Brown Norway rats after elimination of the preexisting Leydig cells
Steroidogenic fate of the Leydig cells that repopulate the testes of young and aged Brown Norway rats after elimination of the preexisting Leydig cells
复制标题
消除先前存在的间质细胞后，在年轻和老年棕色挪威大鼠的睾丸中重新填充间质细胞的类固醇生成命运
DOI：
10.1016/j.exger.2015.08.014
复制
发表时间：
2015-12-01
影响因子：
3.9
通讯作者：
Zirkin, Barry R.
中科院分区：
文献类型：
--
作者：
Chen, Haolin;Guo, Jingjing;Zirkin, Barry R.
褐挪威大鼠间质细胞产生睾酮(T)的能力随着年龄的增长而下降。在之前的研究中，我们报道了单次注射乙烷二甲基磺酸（EDS）后，新一代间质细胞可以在年轻和年老的大鼠睾丸中恢复，并且新一代间质细胞在年轻和年老的大鼠中产生T的能力是相同的。我们在此的目的是比较新的间质细胞的类固醇生成命运随时间的变化。幼龄大鼠（3月龄）和老龄大鼠（18月龄）注射EDS以消除存在的间质细胞。EDS 10周后，间质细胞恢复，从年轻和年老大鼠睾丸中分离的新间质细胞产生的T相同。在对幼鼠进行EDS治疗30周后，新的间质细胞产生T的能力在EDS治疗10周后并没有减弱。相比之下，在eds后30周，老龄大鼠睾丸中新细胞产生的T比10周的水平显著降低。10周和30周血清T水平与间质细胞T生成一致。各组血清LH水平无显著差异。因此，尽管在EDS最初产生等量的高水平T后，年轻和年老的大鼠的间质细胞都恢复了，但年老睾丸中的细胞并没有保持这种能力。这些结果表明：1)产生间质细胞新群体的细胞可能因大鼠年龄的不同而不同；和/或2)年轻和年老睾丸中新形成的间质细胞的外在因素不同，正是这些差异导致老龄大鼠睾丸中新形成的间质细胞的T减少。(C) 2015爱思唯尔公司所有权利
The capacity of Brown Norway rat Leydig cells to produce testosterone (T) decreases with aging. In a previous study, we reported that a new generation of Leydig cells can be restored in both young and old rat testes after a single injection of ethane dimethanesulfonate (EDS), and that the abilities of the new Leydig cells in young and old rats to produce T were equivalent. Our objective herein was to compare the steroidogenic fate of the new Leydig cells over time. Young (3 month-old) and old (18 month-old) rats were injected with EDS to eliminate the existing Leydig cells. Ten weeks after EDS, Leydig cells had been restored and T production by the new Leydig cells isolated from young and old rat testes was equivalent. Thirty weeks after EDS treatment of young rats, the ability of the new Leydig cells to produce T had not diminished from 10 weeks post-EDS. In contrast, at 30 weeks post-EDS, T production by new cells in old rat testes was reduced significantly from the 10-week level. Serum T levels at 10 and 30 weeks were consistent with Leydig cell T production. Serum LH levels did not differ in any group. Thus, although the Leydig cells restored to both young and old rats after EDS initially produced T at high, equivalent levels, the cells in the old testes did not maintain this ability. These results suggest that: 1) the cells from which new populations of Leydig cells are derived may differ depending upon the age of the rat; and/or 2) factors extrinsic to the new Leydig cells in young and old testes differ, and it is these differences that are responsible for reductions in T by the newly formed Leydig cells in the testes of old rats. (C) 2015 Elsevier Inc. All rights