Mesothelin is overexpressed in pancreaticobiliary adenocarcinomas but not in normal pancreas and chronic pancreatitis
Mesothelin is overexpressed in pancreaticobiliary adenocarcinomas but not in normal pancreas and chronic pancreatitis
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DOI：
10.1309/f1b64cl7h8vjkeaf
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发表时间：
2005-12-01
影响因子：
3.5
通讯作者：
Brackett, D
中科院分区：
文献类型：
--
作者：
Hassan, R;Laszik, ZG;Brackett, D
间皮素，一种存在于正常间皮细胞表面的糖蛋白，已被报道在胰腺腺癌中表达。本研究旨在充分表征间皮素在18例胰腺癌、9例壶腹腺癌、12例胆总管腺癌和17例慢性胰腺炎手术切除、福尔马林固定、石蜡包埋组织标本中的表达。间皮素免疫染色采用抗间皮素单克隆抗体5B2。18例胰腺腺癌均有间皮素表达（100%），9例壶腹腺癌中8例（89%）和12例胆总管腺癌均有间皮素表达（100%）。在所有胰胆管腺癌病例中，邻近正常胰腺未见间皮素染色。17例慢性胰腺炎标本中，16例间皮素表达阴性，1例正常胰管间皮素染色弱，低于5%。我们的研究结果表明，间皮素在大多数胰胆腺癌中表达，而在正常胰腺组织和慢性胰腺炎中不表达。
Mesothelin, a cell surface glycoprotein present on normal mesothelial cells, has been reported to be expressed in pancreatic adenocarcinomas. We conducted this study to fully characterize mesothelin expression in surgically resected, formalin-fixed, paraffin-embedded tissue specimens of 18 pancreatic adenocarcinomas, 9 adenocarcinomas of the ampulla of Vater 12 adenocarcinomas of the common bile duct, and 17 cases of chronic pancreatitis. Mesothelin immunostaining was performed using the antimesothelin monoclonal antibody 5B2. All 18 cases (100%) of pancreatic adenocarcinomas showed mesothelin expression, as did 8 (89%) of 9 cases of ampullar adenocarcinoma and all 12 cases (100%) of common bile duct adenocarcinoma. In all cases of pancreaticobiliary adenocarcinoma, the adjacent normal pancreas did not stain for mesothelin. Of 17 specimens of chronic pancreatitis, 16 were negative for mesothelin expression, and 1 case showed weak mesothelin staining of fewer that 5% of normal pancreatic ducts. Our results demonstrated mesothelin expression in the majority of pancreaticobiliary adenocarcinomas and no expression in normal pancreatic tissues and in chronic pancreatitis.