Solid lipid nanoparticles mediate non-viral delivery of plasmid DNA to dendritic cells
Solid lipid nanoparticles mediate non-viral delivery of plasmid DNA to dendritic cells
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DOI：
10.1007/s11051-017-3902-y
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发表时间：
2017-06-09
影响因子：
2.5
通讯作者：
Smooker, Peter M.
中科院分区：
文献类型：
--
作者：
Penumarthi, Alekhya;Parashar, Deepti;Smooker, Peter M.
对新型DNA疫苗递送系统的需求日益增加，主要用于非病毒类型，因为它们被认为相对安全。因此，固体脂质纳米粒（SLN）的适用性进行了研究，作为一个非病毒的DNA疫苗输送系统。SLN的合成通过一种改进的溶剂乳化法，以研究其与质粒DNA的共轭潜力，并将其在体外树突状细胞使用eGFP作为报告质粒。DNA-SLN复合物的特征在于电子显微镜，凝胶阻滞试验和动态光散射。细胞毒性试验数据支持其生物相容性，并用于估计导致高转染率的安全阈值浓度。这些复合物在树突状细胞系中的转染效率与单独的质粒相比显着增加，并且与脂质体介导的转染效率相当。透射电子显微镜研究描绘了细胞摄取的途径。观察到支持转染机制的内体逃逸。
There is an increasing demand for novel DNA vaccine delivery systems, mainly for the non-viral type as they are considered relatively safe. Therefore, solid lipid nanoparticles (SLNs) were investigated for their suitability as a non-viral DNA vaccine delivery system. SLNs were synthesised by a modified solvent-emulsification method in order to study their potential to conjugate with plasmid DNA and deliver them in vitro to dendritic cells using eGFP as the reporter plasmid. The DNA-SLN complexes were characterised by electron microscopy, gel retardation assays and dynamic light scattering. The cytotoxicity assay data supported their biocompatibility and was used to estimate safe threshold concentration resulting in high transfection rate. The transfection efficiency of these complexes in a dendritic cell line was shown to increase significantly compared to plasmid alone, and was comparable to that mediated by lipofectamine. Transmission electron microscopy studies delineated the pathway of cellular uptake. Endosomal escape was observed supporting the mechanism of transfection.