U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.
U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.
复制标题
来自粟酒裂殖酵母的 U1 小核 RNA 具有独特且保守的特征，由必需的单拷贝基因编码。
DOI：
10.1128/mcb.10.6.2874-2881.1990
复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Wise,JA
中科院分区：
文献类型：
--
作者：
Porter,G;Brennwald,P;Wise,JA
我们克隆、测序并破坏了裂殖酵母裂殖酵母U1小核RNA（snRNA）的编码基因。该RNA大小接近，并且与人U1 RNA具有高度的二级结构同源性。S.人U1 RNA;第一个包含参与与5′剪接点氢键结合的核苷酸，第二个是单链区域，在人snRNA中，该区域形成A蛋白结合位点的一部分。pombeU 1在5′帽结构后缺少两个核苷酸，这两个核苷酸存在于迄今为止检测的所有其他U1同源物中，并且对应于人70 K蛋白（分子量为55，000）结合位点的区域比其他生物体中的差异更大。除了U6外，粟酒裂殖酵母中编码剪接体snRNAs的所有基因座中，一个推定的上游转录信号在序列和位置上是保守的。单拷贝U1基因（命名为dsnu 1）的破坏揭示了这种RNA对于生存力是不可或缺的。
We have cloned, sequenced, and disrupted the gene encoding U1 small nuclear RNA (snRNA) in the fission yeastSchizosaccharomyces pombe. This RNA is close in size and exhibits a high degree of secondary structure homology to human U1 RNA. There exist two regions of extended primary sequence identity betweenS. pombeand human U1 RNAs; the first comprises nucleotides involved in hydrogen bonding to 5′ splice junctions, and the second is a single-stranded region which, in the human snRNA, forms part of the A protein binding site.S. pombeU1 lacks two nucleotides just following the 5′ cap structure which are present in all other U1 homologs examined to date, and the region which corresponds to the binding site for the human 70K protein (molecular weight of 55,000) is more divergent than in other organisms. A putative upstream transcription signal is conserved in sequence and location among all loci encoding spliceosomal snRNAs inS.pombewith the exception of U6. Disruption of the single-copy U1 gene, designatedsnu1, reveals that this RNA is indispensable for viability.