DIFFERENT PHYSIOLOGICAL RESPONSES OF FOUR MARINE SYNECHOCOCCUS STRAINS (CYANOPHYCEAE) TO NICKEL STARVATION UNDER IRON‐REPLETE AND IRON‐DEPLETE CONDITIONS 1
DIFFERENT PHYSIOLOGICAL RESPONSES OF FOUR MARINE SYNECHOCOCCUS STRAINS (CYANOPHYCEAE) TO NICKEL STARVATION UNDER IRON‐REPLETE AND IRON‐DEPLETE CONDITIONS 1
复制标题
四种海洋聚球藻菌株（蓝藻科）在富铁和贫铁条件下对镍饥饿的不同生理反应 1
DOI：
10.1111/j.1529-8817.2009.00732.x
复制
发表时间：
2009
影响因子：
2.9
通讯作者：
N. M. Price
中科院分区：
文献类型：
--
作者：
Bao;N. M. Price
聚球藻是沿海和公海生态系统中重要的初级生产者。当以硝酸盐为唯一氮源时，镍饥饿抑制了菌株WH8102和WH7803的生长，而对广盐性菌株WH5701和PCC 7002的生长影响不大。镍是聚球藻WH7803适应低铁强光的必需元素。在WH8102和WH7803中，镍饥饿降低了线性电子传递活性，减缓了QA再氧化，但增加了单个光合单元之间的连接因子。在此条件下，它们的系统间电子传递链的还原作用增强，有利于它们在PSI周围的循环电子传递。镍饥饿使WH8102和WH7803的总超氧化物歧化酶（SOD）活性分别下降了对照的30%和15%。海洋菌株WH8102的蛋白结合的63 Ni与非变性凝胶上的SOD活性共迁移，从而为聚球藻WH8102中存在活性NiSOD提供了额外的证据。在WH7803中，镍饥饿可能会影响其他代谢途径，从而间接影响总SOD活性。
Synechococcus species are important primary producers in coastal and open‐ocean ecosystems. When nitrate was provided as the sole nitrogen source, nickel starvation inhibited the growth of strains WH8102 and WH7803, while it had little effect on two euryhaline strains, WH5701 and PCC 7002. Nickel was required for the acclimation of Synechococcus WH7803 to low iron and high light. In WH8102 and WH7803, nickel starvation decreased the linear electron transport activity, slowed down QA reoxidation, but increased the connectivity factor between individual photosynthetic units. Under such conditions, the reduction of their intersystem electron transport chains was expected to increase, and their cyclic electron transport around PSI would be favored. Nickel starvation decreased the total superoxide dismutase (SOD) activity of WH8102 and WH7803 by 30% and 15% of the control, respectively. The protein‐bound 63Ni of the oceanic strain WH8102 comigrated with SOD activity on nondenaturing gels and thus provided additional evidence for the existence of active NiSOD in Synechococcus WH8102. In WH7803, it seems likely that nickel starvation affected other metabolic pathways and thus indirectly affected the total SOD activity.