FURTHER EVIDENCE FOR THE SELF-REPRODUCING CAPACITY OF LANGERHANS CELLS IN HUMAN-SKIN
FURTHER EVIDENCE FOR THE SELF-REPRODUCING CAPACITY OF LANGERHANS CELLS IN HUMAN-SKIN
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DOI：
10.1111/1523-1747.ep12464659
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发表时间：
1987-01-01
影响因子：
6.5
通讯作者：
DEMARCHEZ, M
中科院分区：
文献类型：
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作者：
CZERNIELEWSKI, JM;DEMARCHEZ, M
表皮中的Langerhans细胞数量有限，是解决LC动力学问题技术困难的主要原因之一。在本文中，我们描述了一种评估表皮中LC复制潜力的方法。该过程基于在细胞周期的S期间，基于胸苷类似物（BRDU）（BRDU）（BRDU）（BRDU）的特异性掺入中。携带人类皮肤移植物的小鼠被注入S.C.每6小时最多与BRDU一起使用17天。在不同的时间，分别使用抗BRDU和OKT-6单克隆抗体在皮肤切片上揭示了掺入的BRDU和人表皮LC。 6小时后，将4.9％的LC标记为BRDU。然后，OKT-6（+）BRDU（+）细胞的数量以线性方式增加，在120 h时达到34％，在240 h时为67％，在连续标记程序过程中400小时在400 h时达到94％。基于此结果，我们计算了人类表皮LC的S期的总细胞周期时间为392 h（16.3天）和12小时。应用这项技术，我们还能够证明，在局部治疗12-O-四烷酰基-13-乙酸酯或剥离后，BRDU标记的LC的数量大大增加了。此外，在I.P.之后在裸小鼠中注射秋水仙碱在移植物中的电子显微镜证明了经历有丝分裂的人表皮LC。从这些结果中，我们得出的结论是，LC正在积极循环 - 在人表皮中有一个自我复制的细胞群体。
The limited number of Langerhans cells (LC) in the epidermis is one of the main reasons for the technical difficulties in resolving the question of LC kinetics. In the present paper, we describe a method to evaluate the LC replication potential in epidermis. The procedure is based on the specific incorporation of bromodeoxyuridine (BrdU), a thymidine analogue, into the DNA during the S-phase of the cell cycle. Mice bearing human skin grafts, were injected s.c. every 6 h for up to 17 days with BrdU. At different times, the incorporated BrdU as well as the human epidermal LC were revealed on skin sections using anti-BrdU and OKT-6 monoclonal antibodies, respectively. After 6 h, 4.9% of the LC were labeled with BrdU. Then, the number of OKT-6(+) BrdU(+) cells increased in a linear manner and achieved 34% at 120 h, 67% at 240 h, and 94% at 400 h during the course of continuous labeling procedures. Based on this results we calculated a total cell cycle time of 392 h (16.3 days) and 12 h for the S-phase for human epidermal LC. Applying this technique, we were able to show also that 48 h after local treatment with 12-O-tetradecanoylphorbol-13-acetate or after stripping, the number of BrdU-labeled Lc was considerably increased. Furthermore, after i.p. injection of colchicine in the nude mouse, human epidermal LC undergoing mitosis were evidenced by electron microscopy in the graft. From these results we conclude that the LC are actively cycling-therewith a self-reproducing cell population in human epidermis.