Acclimation to cold and warm temperatures is associated with differential expression of male carp blood proteins involved in acute phase and stress responses, and lipid metabolism
Acclimation to cold and warm temperatures is associated with differential expression of male carp blood proteins involved in acute phase and stress responses, and lipid metabolism
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DOI：
10.1016/j.fsi.2018.03.018
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发表时间：
2018-05-01
影响因子：
4.7
通讯作者：
Ciereszko, Andrzej
中科院分区：
文献类型：
--
作者：
Dietrich, Mariola A.;Hliwa, Piotr;Ciereszko, Andrzej
环境温度影响鱼类的血浆生化指标、抗氧化状态以及血液学和免疫学参数。到目前为止，只有单一的血液蛋白质被确定为对温度变化的反应。本研究的目的是比较蛋白质组的鲤鱼血浆从男性适应温暖（30摄氏度）和寒冷（10摄氏度）的温度，通过二维差异凝胶电泳，然后MALDI-TOF/TOF质谱。共发现47个蛋白质点受温度差异调节（> 1.2倍变化，p <0.05）：25个蛋白质点在热适应的雄性中更丰富，22个蛋白质点在冷适应的雄性中富集。大多数差异调节的蛋白质与急性期反应信号传导相关，涉及：i）补体系统的激活（补体C3-H1），ii）蛋白水解酶的中和（α间抑制剂H3、胎球蛋白、丝氨酸蛋白酶抑制剂Al、抗凝血酶、α 2-巨球蛋白），iii）清除游离血红蛋白和自由基（触珠蛋白，Wap 65 kDa），iv）凝块形成（纤维蛋白原β和α链，T-激肽原）和v）宿主的免疫应答调节（ApoA 1和ApoA 2）。然而，这些蛋白质或蛋白质型的完全不同的集合参与了对寒冷和温暖温度的反应。此外，冷适应似乎与参与脂质代谢（载脂蛋白A和14 kDa）和应激反应（皮质类固醇结合球蛋白）的蛋白质有关。我们发现了一个在冷驯化过程中受到强烈调控的蛋白Cap 31，它可以作为鲤鱼冷反应的潜在血液生物标志物。这些研究大大扩展了我们对变温动物热适应机制的认识。
The environmental temperature affects plasma biochemical indicators, antioxidant status and hematological and immunological parameters in fish. So far, only single blood proteins have been identified in response to temperature changes. The aim of this study was to compare the proteome of carp blood plasma from males acclimated to warm (30 degrees C) and cold (10 degrees C) temperatures by two-dimensional differential gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. A total of 47 spots were found to be differentially regulated by temperature (> 1.2-fold change, p < 0,05): 25 protein spots were more abundant in warm-acclimated males and 22 were enriched in cold-acclimated males. The majority of differentially regulated proteins were associated with acute phase response signalling involved in: i) activation of the complement system (complement C3-H1), ii) neutralization of proteolytic enzymes (inter-alpha inhibitor H3, fetuin, serpinAl, antithrombin, alpha2-macroglobulin), iii) scavenging of free hemoglobin and radicals (haptoglobin, Wap65 kDa), iv) clot-formation (fibrinogen beta and alpha chain, T-kininogen) and v) the host's immune response modulation (ApoA1 and ApoA2). However, quite different sets of these proteins or proteoforms were involved in response to cold and warm temperatures. In addition, cold acclimation seems to be related to the proteins involved in lipid metabolism (apolipoproteins A and 14 kDa) and stress response (corticosteroid binding globulin). We discovered a strongly regulated protein Cap31 upon cold acclimation, which can serve as a potential blood biomarker of cold response in carp. These studies significantly extend our knowledge concerning mechanisms underlying thermal adaptation in poikilotherms.