Chronologic changes of cerebral ventricular size in a transgenic model of hydrocephalus
Chronologic changes of cerebral ventricular size in a transgenic model of hydrocephalus
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DOI：
10.1159/000055950
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发表时间：
2000-10-01
影响因子：
0.7
通讯作者：
Cohen, AR
中科院分区：
文献类型：
--
作者：
Hayashi, N;Leifer, DW;Cohen, AR
我们建立了一种中枢神经系统过度分泌细胞因子转化生长因子-β 1（transforming growth factor-beta1，TGF-β 1）的转基因小鼠脑积水模型。对胚胎第15天（E15）和第18天（E18）以及出生后4天（P4）小鼠的冠状面切片进行脑室大小的定性分析。通过进行聚合酶链反应（PCR）分析确定TGF-β 1转基因的存在。我们检查了24个胚胎和14个出生后的小鼠。通过PCR分析，在16个（42.1%）胚胎中确定存在TGF-β 1转基因，在E15的16个胚胎中有5个携带转基因，并且显示侧脑室轻微增大，在E18的8个胚胎中有3个携带转基因，并且侧脑室显著增大。P4时14只幼仔中有8只携带转基因，8只携带转基因的幼仔中有7只出现脑积水。在转基因阳性的幼仔中，观察到侧脑室大量扩大，并且存在相关的上覆大脑皮质变薄。这些结果表明，先天性脑积水可能发生在一个重要的胚胎时期，这与中枢神经系统中神经干细胞增殖和分化的阶段相吻合。版权所有（C）2000 S. Karger AG，巴塞尔。
We have maintained a transgenic mouse model of hydrocephalus created to overproduce the cytokine, transforming growth factor-beta1 (TGF-beta1) in the central nervous system (CNS), The aim of the present study was to estimate the embryonic period when the transgenic mice would develop hydrocephalus, by investigating the chronological developmental changes of the cerebral ventricles. Qualitative analysis of ventricular size was performed on sections cut in the coronal plane of embryos at the 15th (E15) and 18th (E18) embryonic days, and postnatal mice aged 4 days (P4). The presence of the TGF-beta1 transgene was determined by performing polymerase chain reaction (PCR) analysis. We have examined 24 embryos and 14 postnatal mice. By performing PCR analysis, the TGF-beta1 transgene was determined to be present in 16 (42.1%), Five of 16 embryos at E15 carried the transgene, and showed a slight enlargement of the lateral ventricles, Three of 8 embryos at E18 carried the transgene, and had remarkable enlargement of the lateral ventricles. Eight of 14 pups at P4 carried the transgene, and 7 of 8 pups with the transgene developed hydrocephalus, In pups that were positive for the transgene, massive enlargement of the lateral ventricles was observed and there was an associated thinning of the overlying cerebral cortex. These results suggest that congenital hydrocephalus may develop at an important embryonic time period, which coincides with the stage of neural stem cell proliferation and differentiation in the CNS. Copyright (C) 2000 S. Karger AG, Basel.