INTRACELLULAR MAGNESIUM DOES NOT ANTAGONIZE CALCIUM-DEPENDENT ACETYLCHOLINE SECRETION
INTRACELLULAR MAGNESIUM DOES NOT ANTAGONIZE CALCIUM-DEPENDENT ACETYLCHOLINE SECRETION
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DOI：
10.1113/jphysiol.1981.sp013705
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发表时间：
1981-01-01
影响因子：
5.5
通讯作者：
SILINSKY, EM
中科院分区：
文献类型：
--
作者：
KHARASCH, ED;MELLOW, AM;SILINSKY, EM
研究了细胞内钙离子和镁离子对蛙运动神经末梢诱发的乙酰胆碱分泌的影响。以脂质体为载体，对神经末梢细胞质进行钙、镁标记。在细胞内应用钙使诱发的ACh分泌成倍增加的条件下，细胞内添加镁不影响诱发的ACh的释放。当镁与钙同时作用于神经末梢细胞质时，ACh的释放进一步增加，高于单独加入钙时的水平。细胞内镁可能不拮抗去极化-分泌偶联，而细胞外镁对递质释放的拮抗作用仅发生在神经末梢的外表面。
The effects of intracellular application of Ca2+ and Mg2+ on evoked acetylcholine [ACh] secretion at frog motor nerve terminals were studied. Ca and Mg were applied to the nerve-ending cytoplasm using liposomes as a vehicle. Under conditions in which intracellular application of Ca produced many-fold increases in evoked ACh secretion, the addition of Mg intracellularly failed to affect evoked ACh release. When Mg was applied to the nerve-ending cytoplasm concurrently with Ca, ACh release was further increased above the level produced by introducing Ca alone. Probably, intracellular Mg does not antagonize depolarization-secretion coupling and antagonism of transmitter release by extracellular Mg occurs only at the external surface of the nerve ending.