Correlation of avian bornavirus-specific antibodies and viral ribonucleic acid shedding with neurological signs and feather-damaging behaviour in psittacine birds
Correlation of avian bornavirus-specific antibodies and viral ribonucleic acid shedding with neurological signs and feather-damaging behaviour in psittacine birds
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禽博纳病毒特异性抗体和病毒核糖核酸脱落与鹦鹉鸟类神经系统症状和羽毛损伤行为的相关性
DOI：
10.1136/vr.104860
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
M. Lierz
中科院分区：
文献类型：
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作者：
A. Fluck;D. Enderlein;A. Piepenbring;U. Heffels;S. Herzog;Kay Pieper;C. Herden;M. Lierz
鹦鹉核糖核酸病毒（PaBV）是鹦鹉热鸟类腺室扩张病的病原体，但也与其他临床体征有关，包括行为障碍和神经体征。本研究的目的是关联PaBV感染的鸟类表现出羽毛损伤行为或神经系统的迹象，没有其他原因的疾病可以确定。将私人执业的鹦鹉鸟分为三组：有神经系统体征的鸟（n=28），显示羽毛损伤行为的鸟（n=42）和常规检查的鸟（n=56）。收集作物和泄殖腔拭子，并使用真实的时间RT-PCR研究PaBV-RNA的存在。另外，采集血清样品并通过免疫荧光试验检查抗PaBV抗体的存在。PaBV感染的检测系统之一，在40.5%的所有鸟类（n=126）的调查。在临床上健康的鸟类（n=56），19.6%的鸟类在至少一个PaBV测试中呈阳性，而羽毛损伤（n=42）和64.28%的神经系统疾病的鸟类（n=28）。有趣的是，具有神经系统体征的鸟类中的抗PaBV抗体滴度最高达1：20 480。在羽毛损伤组中也发现了高抗体滴度（高达1：5120），而对照组的鸟，如果PaBV阳性，则仅具有非常低的滴度。同样，最高的病毒载量被发现在组中的神经系统疾病的鸟类，其次是羽毛损伤的鸟类，而在对照组中的PaBV阳性的鸟类表现出只有低病毒RNA脱落。对于大多数神经系统疾病的鸟类和少数具有羽毛损伤行为的鸟类，观察到临床体征的严重程度、病毒脱落量和高水平抗体滴度之间存在明显的相关性。这些结果第一次清楚地表明，在实践中没有向兽医提出胃肠道体征的鸟类中，PaBV感染与神经系统体征之间存在相关性。它也可能证明与羽毛损伤行为和抗PaBV抗体存在可能的相关性。抗体滴度似乎代表了将临床体征与PaBV作为病因相关联的诊断工具。
Parrot bornaviruses (PaBV) are the causative agents of proventricular dilatation disease in psittacine birds, but have also been linked to other clinical signs, including behavioural disorders and neurological signs. The aim of this study was to correlate PaBV infection in birds showing feather-damaging behaviour or neurological signs for which no other cause of disease could be identified. Psittacine birds presented to a private practice were divided into three groups: birds with neurological signs (n=28), birds showing feather-damaging behaviour (n=42) and birds presented for routine examinations (n=56). Swabs of crop and cloaca were collected and investigated for the presence of PaBV-RNA using real time RT-PCR. Additionally, serum samples were taken and examined for the presence of anti-PaBV antibodies by immunofluorescence test. PaBV infection was detected in one of the test systems in 40.5 per cent of all birds (n=126) investigated. In the clinically healthy birds (n=56), 19.6 per cent of the birds were positive in at least one of the PaBV tests, compared with 52.38 per cent of the feather-damaging (n=42) and 64.28 per cent of the neurologically diseased birds (n=28). Interestingly, the anti-PaBV antibody titres in birds with neurological signs were highest up to 1:20 480. High antibody titres (up to 1:5120) were also found in the feather-damaging group, whereas the birds of the control group, if PaBV positive, had only very low titres. Similarly, the highest viral load was found in the group of the neurologically diseased birds, followed by feather-damaging birds, whereas PaBV-positive birds in the control group demonstrated only low viral RNA shedding. A clear correlation between severity of clinical signs, amount of viral shedding and high levels of antibody titres was observed for most of the neurologically diseased birds and also for few birds with feather-damaging behaviour. For the first time, these results clearly indicate a correlation between PaBV infection and neurological signs in birds without gastrointestinal signs presented to the veterinarian in practice. It also may demonstrate a possible correlation with feather-damaging behaviour and anti-PaBV antibody presence. The antibody titre seems to represent a diagnostic tool to correlate clinical signs to PaBV as a cause.