The Formation of the Duct in Xenopus Involves Recruitment of Posterior Cells by Migrating Pronephric Duct Cells
The Formation of the Duct in Xenopus Involves Recruitment of Posterior Cells by Migrating Pronephric Duct Cells
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爪蟾导管的形成涉及通过迁移前肾管细胞招募后部细胞
DOI：
10.1006/dbio.1993.1245
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发表时间：
1993
影响因子：
2.7
通讯作者：
L. Etkin
中科院分区：
文献类型：
--
作者：
J. A. Cornish;L. Etkin
细胞迁移和细胞间相互作用在胚胎发生过程中的许多过程中发挥着重要作用。其中之一是前肾（沃尔夫）管（PD）的形成，它将前肾与泄殖腔连接起来。目前人们普遍认为，在大多数两栖动物中，前肾管是由前肾管雏形（PDR）细胞沿着预定途径主动迁移而形成的。然而，有证据表明，在非洲爪蟾中，PD 可能完全是通过细胞从外侧中胚层原位分离而形成的。在这项研究中，我们使用 PDR 消融和非洲爪蟾-北极爪蟾嵌合体证明，非洲爪蟾的 PD 伸长需要主动细胞迁移和诱导后部细胞的招募。我们还表明，PDR 细胞迁移仅限于发育过程中的几个阶段，并且这种时间控制至少部分归因于 PD 途径支持细胞迁移能力的变化。
Cell migration and cell-cell interactions play important roles in numerous processes during embryogenesis. One of these is the formation of the pronephric (Wolffian) duct (PD), which connects the pronephros to the cloaca. It is currently accepted that in most amphibians the pronephric duct is formed by active migration of the pronephric duct rudiment (PDR) cells along a predetermined pathway. However, there is evidence that in Xenopus, the PD may be formed entirely by in situ segregation of cells out of the lateral mesoderm. In this study, we show, using PDR ablation and Xenopus laevis-Xenopus borealis chimeras, that PD elongation in Xenopus requires both active cell migration and an induced recruitment of cells from the posterior. We also show that PDR cell migration is limited to only a few stages during development and that this temporal control is due, at least in part, to changes in the competence of the PD pathway to support cell migration.