Roburic Acid Suppresses NO and IL-6 Production via Targeting NF-κB and MAPK Pathway in RAW264.7 Cells
Roburic Acid Suppresses NO and IL-6 Production via Targeting NF-κB and MAPK Pathway in RAW264.7 Cells
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Roburic Acid 通过靶向 RAW264.7 细胞中的 NF-kB 和 MAPK 通路抑制 NO 和 IL-6 的产生
DOI：
10.1007/s10753-017-0636-z
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发表时间：
2017-12-01
期刊：
影响因子：
5.1
通讯作者：
Kong, Dexin
中科院分区：
文献类型：
--
作者：
Chen, Yufen;Ji, Ning;Kong, Dexin
在本研究中，我们研究了罗布酸对脂多糖（LPS）刺激的RAW 264.7巨噬细胞产生一氧化氮（NO）和白细胞介素-6（IL-6）的抗炎作用。我们发现，罗布酸减少NO和IL-6的产生，诱导型一氧化氮合酶（iNOS）的表达。同时，罗布酸处理抑制了κ B α（I kappa B α）和I kappa B激酶α/β（IKK α/β）抑制剂的磷酸化，以及核因子-κ B（NF-κ B）向细胞核的易位。此外，包括p38和c-Jun-NH 2-末端激酶（JNK）在内的丝裂原活化蛋白激酶（MAPK）的磷酸化被抑制。Roburic acid通过阻断IKK/I kappa B/NF-κ B和MAPK途径抑制NO和IL-6的产生，有望成为治疗炎症性疾病的候选药物。
In the present study, we investigated the anti-inflammatory effect of roburic acid on production of nitric oxide (NO) and interlukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. We found that roburic acid reduced production of NO and IL-6, and the expression of inducible nitric oxide synthases (iNOS). Meanwhile, phosphorylation of inhibitor of kappa B alpha (I kappa B alpha) and I kappa B kinase alpha/beta (IKK alpha/beta), as well as translocation of nuclear factor-kappa B (NF-kappa B) to the nucleus, was suppressed by roburic acid treatment. In addition, phosphorylation of mitogen-activated protein kinase (MAPKs) including p38 and c-Jun-NH2-terminal kinase (JNK) was inhibited. Roburic acid exhibited inhibitory activities on production of NO and IL-6 via blocking IKK/I kappa B/NF-kappa B and MAPKs pathway, suggesting the potential application as a drug candidate for therapy of inflammatory diseases.