LOW-TEMPERATURE ACCUMULATION OF ALCOHOL DEHYDROGENASE-1 MESSENGER-RNA AND PROTEIN-ACTIVITY IN MAIZE AND RICE SEEDLINGS
LOW-TEMPERATURE ACCUMULATION OF ALCOHOL DEHYDROGENASE-1 MESSENGER-RNA AND PROTEIN-ACTIVITY IN MAIZE AND RICE SEEDLINGS
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DOI：
10.1104/pp.95.3.699
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发表时间：
1991-03-01
期刊：
影响因子：
7.4
通讯作者：
WALBOT, V
中科院分区：
文献类型：
--
作者：
CHRISTIE, PJ;HAHN, M;WALBOT, V
低温胁迫导致玉米（Zea mays）（B37 N，A188）和水稻（Oryza sativa）（Taipei 309，Calmochi 101）幼苗中乙醇脱氢酶-1信使（Adh 1）和蛋白质活性（ADH 1）的稳态水平迅速增加。 玉米根和水稻芽和根从7天的幼苗转移到低温（10 ℃）含有多达15倍以上的Adh 1 mRNA和8倍以上的ADH 1蛋白活性比相应的组织从未处理的幼苗。 时间进程研究表明，这些组织积累的Adh 1 mRNA和ADH 1活性在4至8小时内的几倍，水平在20至24小时内达到平台，并保持在4天的冷处理升高。 在24小时内返回冷胁迫幼苗到环境温度，Adh 1 mRNA和ADH 1活性下降到预处理水平。 对玉米和水稻组织印迹的组织化学染色结果表明，沿着低温胁迫下，玉米初生根和水稻根、水稻茎、叶沿着的ADH活性均增强。 我们的观察结果表明，短期冷胁迫诱导Adh 1基因在某些植物组织中的表达，这，回忆的厌氧反应，可能反映了能量代谢的根本转变，以确保在胁迫期间组织的生存。
Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice (Oryza sativa) (Taipei 309, Calmochi 101) seedlings. Maize roots and rice shoots and roots from 7-day seedlings shifted to low temperature (10-degrees-C) contained as much as 15-fold more Adh1 mRNA and 8-fold more ADH1 protein activity than the corresponding tissues from untreated seedlings. Time-course studies showed that these tissues accumulated Adh1 mRNA and ADH1 activity severalfold within 4- to 8-hour, levels plateaued within 20 to 24 hours, and remained elevated at 4 days of cold treatment. Within 24 hours of returning cold-stressed seedlings to ambient temperature, Adh1 mRNA and ADH1 activity decreased to pretreatment levels. Histochemical staining of maize and rice tissue imprints showed that ADH activity was enhanced along the lengths of cold-stressed maize primary roots and rice roots, and along the stems and leaves of rice shoots. Our observations suggest that short-term cold stress induces Adh1 gene expression in certain plant tissues, which, reminiscient of the anaerobic response, may reflect a fundamental shift in energy metabolism to ensure tissue survival during the stress period.