Recombinant human MCP-1/JE induces chemotaxis, calcium flux, and the respiratory burst in human monocytes.
Recombinant human MCP-1/JE induces chemotaxis, calcium flux, and the respiratory burst in human monocytes.
复制标题
DOI：
10.1182/blood.v78.4.1112.bloodjournal7841112
复制
发表时间：
1991-08
期刊：
影响因子：
20.3
通讯作者：
B. Rollins;A. Walz;M. Baggiolini
中科院分区：
文献类型：
--
作者：
B. Rollins;A. Walz;M. Baggiolini
JE基因首先被描述为小鼠3 T3细胞中的血小板衍生生长因子（PDGF）诱导基因。JE的人类同源物编码一种蛋白质，其预测的氨基酸序列与单核细胞趋化因子MCP-1（也称为MCAF和SMC-CF）的氨基酸序列相同，MCP-1属于最近鉴定的具有细胞因子特性的小分泌蛋白家族。我们纯化了在COS细胞中产生的重组人MCP-1/JE（hMCP-1/JE），并证明其对人单核细胞具有趋化性，具有与天然MCP-1相似的特异性活性。此外，纯的重组hMCP-1/JE刺激单核细胞，诱导细胞溶质游离钙和呼吸爆发的增加，但对人中性粒细胞完全无活性。这些结果有助于在功能上定义一个众所周知的生长因子诱导基因和一个新的细胞因子家族的成员。
The JE gene was first described as a platelet-derived growth factor (PDGF)-inducible gene in mouse 3T3 cells. The human homologue of JE encodes a protein whose predicted amino acid sequence is identical to that of the monocyte chemoattractant MCP-1 (also called MCAF and SMC-CF), which belongs to a recently identified family of small secretory proteins with cytokine properties. We purified recombinant human MCP-1/JE (hMCP-1/JE) produced in COS cells and demonstrated that it is chemotactic for human monocytes with a specific activity similar to natural MCP-1. In addition, pure recombinant hMCP-1/JE stimulates monocytes, inducing an increase in cytosolic free calcium and the respiratory burst, but is completely inactive on human neutrophils. These results help to define functionally a well-known growth factor-inducible gene and a member of a new family of cytokines.