Regulation of three beta‐tubulin mRNAs during rat brain development.
Regulation of three beta‐tubulin mRNAs during rat brain development.
复制标题
大鼠大脑发育过程中三种 β-微管蛋白 mRNA 的调节。
DOI：
10.1002/j.1460-2075.1985.tb04133.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
U. Littauer
中科院分区：
文献类型：
--
作者：
I. Ginzburg;A. Teichman;H. Dodemont;L. Behar;U. Littauer
完整的大鼠脑 β-微管蛋白 T β 15 的核苷酸序列已从三个重叠的 cDNA 克隆中确定。 T beta 15 序列的总长度为 1589 bp，与鸡和人 β-微管蛋白序列相比，编码区内的同源性为 84.5% 至 88.6%。另一方面，3′非编码区差异很大。来自不同物种的衍生氨基酸序列的比较表明，氨基酸变化不是随机分布的，而是来自不同来源的β-微管蛋白多肽共有几个保守区和两个高度可变的区域。 T beta 15 序列编码主要神经元 1.8 kb β-微管蛋白 mRNA 种类。大鼠大脑中还存在另外两种 2.6 和 2.9 kb 的小 β-微管蛋白 mRNA 种类。通过使用与羧基末端分歧区域和氨基末端保守区域互补的两个合成寡核苷酸探针，我们已经证明这三种 mRNA 是不同的物种，它们受到发育调控。 1.8-kb mRNA 种类的水平增加，直到 12 天龄，此后其水平下降。 2.9-kb mRNA 是早期神经元 mRNA 种类，而 2.6-kb mRNA 是晚期神经元种类，在大鼠大脑发育 30 天时检测到。数据表明，在大鼠大脑发育过程中，β-微管蛋白多基因家族存在差异表达，这可能表明各种β-微管蛋白同位素具有不同的功能。
The nucleotide sequence of a complete rat brain beta‐tubulin T beta 15 has been determined from three overlapping cDNA clones. The overall length of the T beta 15 sequence is 1589 bp and shows between 84.5% and 88.6% homology within the coding region as compared with chick and human beta‐tubulin sequences. On the other hand, the 3′‐non‐coding region is highly divergent. Comparison of the derived amino acid sequences from different species demonstrates that the amino acid changes are not randomly distributed, but rather there are several conserved and two highly variable regions common to beta‐tubulin polypeptides from various sources. The T beta 15 sequence encodes a dominant neuronal 1.8‐kb beta‐tubulin mRNA species. Two other minor beta‐tubulin mRNA species of 2.6 and 2.9 kb are present in rat brain. By using two synthetic oligonucleotide probes complementary to the carboxyl‐terminal divergent region and to the amino‐terminal conserved region, we have shown that the three mRNAs are distinct species, which are developmentally regulated. The level of the 1.8‐kb mRNA species increases till the age of 12 days thereafter its level decreases. The 2.9‐kb mRNA is an early neuronal mRNA species, while the 2.6‐kb mRNA is a late neuronal species which is detected at 30 days of rat brain development. The data illustrate that there is a differential expression of the beta‐tubulin multigene family during rat brain development which may suggest different functions for the various beta‐tubulin isotopes.