A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3T3 proliferation.
A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3T3 proliferation.
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发表时间：
1992-10
期刊：
影响因子：
11.2
通讯作者：
Benigno C. Valdez;Laszlo Perlaky;Yasuo Saijo;D. Henning;Cihui Zhu;Rose K. Busch;Wei-Wei Zhang-Wei;H. Busch
中科院分区：
文献类型：
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作者：
Benigno C. Valdez;Laszlo Perlaky;Yasuo Saijo;D. Henning;Cihui Zhu;Rose K. Busch;Wei-Wei Zhang-Wei;H. Busch
人核仁p120蛋白是一种增殖相关抗原，在G1期表达，在细胞周期的早期S期达到峰值。人p120蛋白的过表达引起NIH 3 T3细胞的转化，而反义p120构建体的表达抑制NIH 3 T3细胞的生长（Perlaky等，癌症研究所，52：428-436，1992）。发现反义p120 RNA的中间区域几乎与全长反义构建体一样具有抑制作用，但5'和3'反义部分不影响NIH 3 T3细胞增殖。小鼠p120互补DNA克隆并测序后，与人p120互补DNA比较，显示85%的核苷酸序列和96%的氨基酸序列具有惊人的保守性。p120分子两端的核苷酸和氨基酸序列同源性较低。基于这种同源性，观察到的抑制作用的反义人p120 RNA的中间部分可能与抑制小鼠p120表达的RNA：RNA双链体的形成。中间区域的高度进化保守性表明它对该蛋白质的功能具有关键作用。
The human nucleolar p120 protein is a proliferation-associated antigen which is expressed in G1 and peaks during the early S phase of the cell cycle. Overexpression of the human p120 protein caused the transformation of NIH 3T3 cells and expression of an antisense p120 construct inhibited the growth of NIH 3T3 cells (Perlaky et al., Cancer Res., 52:428-436, 1992). The middle region of the antisense p120 RNA was found to be almost as inhibitory as the full length antisense construct but the 5' and 3' antisense portions did not affect NIH 3T3 cell proliferation. After the mouse p120 complementary DNA was cloned and sequenced, comparison with the human p120 complementary DNA showed a striking conservation of 85% of the nucleotide sequence and 96% of the amino acid sequence. The two ends of the p120 molecule had less homology in their nucleotide and amino acid sequences. Based on this homology, the observed inhibitory effects of the middle portion of antisense human p120 RNA may be related to suppression of mouse p120 expression by RNA:RNA duplex formation. The high evolutionary conservation of the middle region suggests it has a critical role for the function of this protein.