Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies
Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies
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DOI：
10.1002/bmc.980
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发表时间：
2008-06-01
影响因子：
1.8
通讯作者：
Nakashima, Kenichiro
中科院分区：
文献类型：
--
作者：
Ikeda, Rie;Nishimura, Miho;Nakashima, Kenichiro
建立了一种简便的HPLC-UV法，并结合腺苷、虫草素、2 '-脱氧腺苷、鸟苷和尿苷的提取方法，用于冬虫夏草及其近缘植物的鉴别。采用C-18柱，乙腈-水等度洗脱，紫外检测波长260 nm。虫草中腺苷、虫草素、2 ′-脱氧腺苷、鸟苷和尿苷的含量分别为0.28-14.15、0.006-6.36、0.01-0.14、0.68-14.79和0.19-20.29 mg/g。在所研究的核苷类化合物中，蛹虫草素是蛹虫草中的特征性成分。链接. (CM)，是冬虫夏草的重要类群之一，可作为冬虫夏草的鉴别标记。虫草中核苷与腺苷的比值可作为虫草鉴别和质量控制的指标。版权所有（C）2008约翰威利父子有限公司
A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C-18 column by isocratic elution with acetonitrile-water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28-14.15, 0.006-6.36, 0.01-0.14, 0.68-14.79 and 0.19-20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps. Copyright (C) 2008 John Wiley & Sons, Ltd.