Serum microRNA expression profile as a diagnostic panel for gastric cancer
Serum microRNA expression profile as a diagnostic panel for gastric cancer
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血清 MicroRNA 表达谱作为胃癌的诊断依据
DOI：
10.1093/jjco/hyw085
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发表时间：
2016-09-01
影响因子：
2.4
通讯作者：
Huang, Changzhi
中科院分区：
文献类型：
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作者：
Huang, Shengkai;Wang, Jia;Huang, Changzhi
目的：以前，我们鉴定了6种在结直肠癌中与健康对照相比差异表达的miRNAs。方法：我们对92例胃癌患者和89例正常人的血清样本进行定量RT-PCR检测，并分析其风险评分，以评估血清miRNA谱系统的诊断价值。结果：经过两个阶段的选择和验证过程，发现5种miRNA在胃癌样品和对照样品之间的表达显著不同，包括miR-21、miR-31、miR-92 a、miR-181 b和miR-203。风险评分分析表明，该miRNA组可以区分胃癌病例和对照组，具有较高的敏感性和特异性。在受试者工作特征曲线下，肿瘤识别的曲线下面积为0.933（95%置信区间[CI]：0.86-1.007）和0.919（95%CI：0.863-0.975）-显著高于癌胚抗原结论：这5种miRNAs是一种新的、非侵袭性的胃癌生物标志物，可用于胃癌的诊断。
Purpose: Previously, we identified six miRNAs that are differentially expressed in colorectal cancer compared with healthy controls. Here, we tested them in gastric cancer GC.Methods: We performed quantitative RT-PCR on serum samples from 92 patients with gastric cancer and 89 controls for the six miRNAs, and analyzed their risk scores to evaluate the diagnostic value of the serum miRNA profiling system.Results: After a two-phase selection and validation process, five miRNAs were found to significantly differ in expression between gastric cancer samples and control samples, including miR-21, miR-31, miR-92a, miR-181b, and miR-203. Risk score analysis showed that this miRNA panel could distinguish gastric cancer cases from controls with high sensitivity and specificity. Under receiver operating characteristic curves, areas under the curve for tumor identification were 0.933 (95% confidence interval [CI]: 0.86-1.007) for the training set and 0.919 (95% CI: 0.863-0.975) for the validation set-markedly higher than those of carcinoembryonic antigen (0.624) and carbohydrate antigen 19-9 (0.603).Conclusions: The signature of these five miRNAs is a novel and noninvasive biomarker for gastric cancer, and could facilitate and simplify its diagnosis.