Moina macrocopa as a non-target aquatic organism for assessment of ecotoxicity of silver nanoparticles: Effect of size
Moina macrocopa as a non-target aquatic organism for assessment of ecotoxicity of silver nanoparticles: Effect of size
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DOI：
10.1016/j.chemosphere.2018.12.031
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发表时间：
2019-03-01
期刊：
影响因子：
8.8
通讯作者：
Singhal, Rekha S.
中科院分区：
文献类型：
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作者：
Borase, Hemant P.;Patil, Satish, V;Singhal, Rekha S.
纳米材料在水库中的释放是危险的。关于不同大小的纳米颗粒与水生生物和水生环境相互作用的报道很少。本研究以聚乙烯吡咯烷酮和l -酪氨酸为原料合成了平均粒径为20.80 +/- 2.31 nm和40.04 +/- 4.72 nm的银纳米粒子（AgNPs）。对AgNPs的生态毒理学效应进行了评价。20和40 nm AgNPs的48 h致死值（48 h LC50）分别为0.11 +/- 0.02和0.12 +/- 0.03 mg/L。此外，AgNPs对乙酰胆碱酯酶和消化酶（胰蛋白酶、淀粉酶、β -半乳糖苷酶）的抑制作用呈大小依赖性，对过氧化氢酶、超氧化物歧化酶、谷胱甘肽- s -转移酶)和碱性磷酸酶的抑制作用较对照组增强。这些结果加强了酶作为AgNPs环境风险评估生物标志物的潜力。AgNPs在M. macrocopa的肠道中积累，无法完全消除，从而增加了金属体负担。粒径为20 nm的AgNPs蓄积量低于粒径为40 nm的AgNPs蓄积量，说明粒径对AgNPs的吸收和毒性有影响。AgNPs在中等硬水介质（MHWM）中聚集，这种聚集影响了生物体对其的暴露。AgNPs的大小通过在生物体和环境中吸收、积累、聚集和排泄的相互作用影响对巨巨单胞菌的毒性。(C) 2018 Elsevier Ltd.版权所有。
The release of nanomaterials in water reservoirs is hazardous. Very few reports are available on the interaction of different sized nanoparticles with aquatic organisms and aquatic environment. In the present study, silver nanoparticles (AgNPs) having an average particle size of 20.80 +/- 2.31 and 40.04 +/- 4.72 nm were synthesized using polyvinylpyrrolidone and L-tyrosine. Ecotoxicological effects of AgNPs were evaluated on less explored crustacean species, Moina macrocopa. The 48 h lethal values (48 h LC50) of 20 and 40 nm AgNPs were 0.11 +/- 0.02 and 0.12 +/- 0.03 mg/L, respectively. Further, a size dependent inhibition of AgNPs on acetyl cholinesterase and digestive enzymes (trypsin, amylase, beta-galactosidase) was observed, while that of the antioxidant enzymes (catalase, superoxide dismutase, glutathione-S-transferase) and alkaline phosphatase were enhanced as compared to control group. These results strengthen the potential of enzymes as biomarker in environmental risk assessment of AgNPs. AgNPs accumulated in the gut of M. macrocopa which could not be completely eliminated, thereby resulting in an increased metal body burden. The accumulation of AgNPs of 20 nm was lower than that of 40 nm indicating the influence of size of nanoparticles on uptake and toxicity. AgNPs agglomerated in moderately hard water medium (MHWM) and this agglomeration influenced the exposure the organism thereto. The size of AgNPs influenced the toxicity to M. macrocopa through interplay between uptake, accumulation, aggregation, and excretion in the organism and environment. (C) 2018 Elsevier Ltd. All rights reserved.