Microscale nonlinear electrokinetics for the analysis of cellular materials in clinical applications: a review
Microscale nonlinear electrokinetics for the analysis of cellular materials in clinical applications: a review
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DOI：
10.1007/s00604-021-04748-7
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发表时间：
2021-03
影响因子：
5.7
通讯作者：
B. Lapizco-Encinas
中科院分区：
文献类型：
--
作者：
B. Lapizco-Encinas
本文综述了微尺度电动力学在细胞和亚细胞材料分析领域的最新进展。引言概述了电场的使用，即，电动力学，在微流体设备，并讨论了潜在的电动为基础的方法分析液体活检在临床和护理点的应用。接下来是四个综合部分，介绍了循环肿瘤细胞、血液（红细胞、白色细胞和血小板）、干细胞和亚细胞颗粒（细胞外囊泡和线粒体）分析的一些最新发现。这里讨论的有价值的贡献（131篇参考文献）主要是在过去的3至4年中发表的，为读者提供了在临床分析中使用微尺度电动方法的最新技术的概述。最后，在结论部分总结了本文的主要研究成果，并对未来的研究方向进行了展望。
This review article presents a discussion of some of the latest advancements in the field of microscale electrokinetics for the analysis of cells and subcellular materials in clinical applications. The introduction presents an overview on the use of electric fields, i.e., electrokinetics, in microfluidics devices and discusses the potential of electrokinetic-based methods for the analysis of liquid biopsies in clinical and point-of-care applications. This is followed by four comprehensive sections that present some of the newest findings on the analysis of circulating tumor cells, blood (red blood cells, white blood cells, and platelets), stem cells, and subcellular particles (extracellular vesicles and mitochondria). The valuable contributions discussed here (with 131 references) were mainly published during the last 3 to 4 years, providing the reader with an overview of the state-of-the-art in the use of microscale electrokinetic methods in clinical analysis. Finally, the conclusions summarize the main advancements and discuss the future prospects.Graphical abstract