Gibberellins are involved in effect of near‐null magnetic field on Arabidopsis flowering
Gibberellins are involved in effect of near‐null magnetic field on Arabidopsis flowering
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DOI：
10.1002/bem.22004
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发表时间：
2017-01
影响因子：
1.9
通讯作者：
Chunxiao Xu;Yang Yu;Yuxia Zhang;Yue Li;Shufeng Wei
中科院分区：
文献类型：
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作者：
Chunxiao Xu;Yang Yu;Yuxia Zhang;Yue Li;Shufeng Wei
我们以前发现拟南芥的开花受到近零磁场的抑制，这与隐花色素的修饰有关。为了揭示这种效应的生理机制，我们检测了在近零磁场下生长的野生型拟南芥和隐花色素双突变体cry 1/cry 2的赤霉素（GA）水平以及GA生物合成和信号基因的表达。我们发现，与当地地磁场控制相比，近零磁场中野生型植物的GA 4，GA 9，GA 34和GA 51水平显着降低。然而，在近零磁场中，cry 1/cry 2突变体中的GA水平与对照相似。三种GA 20-氧化酶（GA 20 ox）基因的表达（GA 20 ox 1、GA 20 ox 2和GA 20 ox 3）和4个GA 3-氧化酶（GA 3 ox）基因与对照相比，近零磁场中野生型植物中的GA 3 ox 1、GA 3 ox 2、GA 3 ox 3和GA 3 ox 4基因的表达显著降低，而GA 20 ox 4、GA 20 ox 5、GA 2氧化酶（GA 2 ox）基因的表达，近零磁场中野生型植物中GA信号相关基因与对照无显著差异。相反，在cry 1/cry 2突变体中检测到的所有GA生物合成和信号传导基因的表达不受近零磁场的影响。此外，开花相关基因LFY和SOC 1在野生型植物中的转录被近零磁场下调，而在cry 1/cry 2突变体中，它们不受近零磁场的影响。我们的研究结果表明，近零磁场对拟南芥开花的影响是GA相关的，这是由隐花色素参与的GA生物合成抑制引起的。生物电磁学38：1-10，2017.© 2016 Wiley Periodicals，Inc.
We previously found that flowering of Arabidopsis was suppressed by near‐null magnetic field, which was related to the modification of cryptochrome. To disclose the physiological mechanism of this effect, we detected gibberellin (GA) levels and expressions of GA biosynthetic and signaling genes in wild type Arabidopsis plants and cryptochrome double mutant, cry1/cry2, grown in near‐null magnetic field. We found that levels of GA4, GA9, GA34, and GA51 in wild type plants in near‐null magnetic field were significantly decreased compared with local geomagnetic field controls. However, GA levels in cry1/cry2 mutants in near‐null magnetic field were similar to controls. Expressions of three GA20‐oxidase (GA20ox) genes (GA20ox1, GA20ox2, and GA20ox3) and four GA3‐oxidase (GA3ox) genes (GA3ox1, GA3ox2, GA3ox3, and GA3ox4) in wild type plants in near‐null magnetic field were significantly reduced compared with controls, while expressions of GA20ox4, GA20ox5, GA2‐oxidase (GA2ox) genes, and GA signaling‐related genes in wild type plants in near‐null magnetic field were not significantly different from controls. In contrast, expressions of all the detected GA biosynthetic and signaling genes in cry1/cry2 mutants were not affected by near‐null magnetic field. Moreover, transcriptions of flowering‐related genes, LFY and SOC1, in wild type plants were downregulated by near‐null magnetic field, while they were not affected by near‐null magnetic field in cry1/cry2 mutants. Our results suggest that the effect of near‐null magnetic field on Arabidopsis flowering is GA‐related, which is caused by cryptochrome‐involved suppression of GA biosynthesis. Bioelectromagnetics. 38:1–10, 2017. © 2016 Wiley Periodicals, Inc.