Stimulation of dome formation in MDCK kidney epithelial cultures by inducers of differentiation: dissociation from effects on transepithelial resistance and cyclic AMP levels.
Stimulation of dome formation in MDCK kidney epithelial cultures by inducers of differentiation: dissociation from effects on transepithelial resistance and cyclic AMP levels.
复制标题
分化诱导剂刺激 MDCK 肾上皮培养物中圆顶形成：与跨上皮阻力和环 AMP 水平的影响分离。
DOI：
10.1002/jcp.1041130311
复制
发表时间：
1982
影响因子：
5.6
通讯作者：
Lever,JE
中科院分区：
文献类型：
--
作者：
Thomas,SR;Schultz,SG;Lever,JE
监测跨上皮电阻和环核苷酸水平的变化，伴随着MDCK肾上皮细胞克隆亚系中圆顶的化学诱导。在硝酸纤维素滤膜上生长的融合细胞单层表现出相对较高的平均跨上皮电阻（387 ohms ·cm 2）。六亚甲基二乙酰胺是一种有效的圆顶形成诱导剂（Lever，1979 b），可刺激跨单层阻力显著增加以及细胞内环AMP水平升高。相比之下，二甲基甲酰胺，一个同样有效的诱导剂圆顶形成MDCK细胞，没有明显改变电阻值或环核苷酸水平。这些结果表明，通常称为分化诱导剂的化合物诱导上皮细胞培养物中的圆顶形成可能涉及多种不同的机制。
Changes in transepithelial electrical resistance and cyclic nucleotide levels were monitored accompanying chemical induction of domes in a clonal subline of MDCK kidney epithelial cells. Confluent cell monolayers grown on nitrocellulose filters exhibited a relatively high mean transepithelial resistance (387 ohms · cm2). Hexamethylene bisacetamide, a potent inducer of dome formation (Lever, 1979b), stimulated significantly increased transmonolayer resistance as well as elevated levels of intracellular cyclic AMP. By contrast, dimethylformamide, an equally potent inducer of dome formation in MDCK cells, did not appreciably alter either resistance values or cyclic nucleotide levels. These results suggest that induction of dome formation in epithelial cell cultures by compounds generally known as inducers of differentiation may involve multiple and separate mechanisms.