Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)
Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)
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DOI：
10.1007/s10327-020-00928-0
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发表时间：
2020-05
影响因子：
1.2
通讯作者：
Muhammad Akhid Syib’li;Aoi Kodama;Ayumi Abe;T. Sone
中科院分区：
文献类型：
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作者：
Muhammad Akhid Syib’li;Aoi Kodama;Ayumi Abe;T. Sone
线粒体是QOI抗真菌药物的靶细胞器，但线粒体在稻瘟病菌中的行为尚不清楚。利用激光扫描共聚焦显微镜和计算机分析技术，对转化柠檬酸合成酶(CITA)-GFP融合基因真菌分离株的分生孢子线粒体进行了原位观察。在36h收获菌丝并去除气生菌丝后，随着时间的推移，观察到带有1、2或3个细胞的分生孢子。这些分生孢子内线粒体的三维可视化显示，随着分生孢子的老化，线粒体最初呈点状，并发展为网络状。
The mitochondrion is the target organelle for QoI (quinone outside inhibitor) antifungal drugs, but mitochondrial behavior inPyricularia oryzaeremains unclear. Conidial mitochondria in fungal isolate transformed with citrate synthase (CitA)-GFPfusion gene, were observed in situ using laser-scanning confocal microscopy and computer analysis. After mycelium was harvested at 36 h and aerial mycelia removed, conidia with 1, 2, or 3 cells were observed over time. The 3D visualization of mitochondria in these conidia showed that mitochondria were initially shaped like dots and developed into a network as the conidia aged.