LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
LH supplementation of ovarian stimulation protocols influences follicular fluid steroid composition contributing to the improvement of ovarian response in poor responder women
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DOI：
10.1038/s41598-020-69325-z
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发表时间：
2020-07-31
影响因子：
4.6
通讯作者：
Baldi, E.
中科院分区：
文献类型：
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作者：
Marchiani, S.;Tamburrino, L.;Baldi, E.
在这项前瞻性研究中，我们评估了收集的111个卵泡液（FF）中的类固醇水平，这些卵泡液来自13名接受FSH单药治疗的女性，而收集的205个卵泡液来自28名接受FSH+LH刺激的女性，因为她们对FSH的反应性较低。通过HPLC/MS-MS测量类固醇水平，并在单独跟踪所有检索到的卵母细胞的命运后，将类固醇水平与卵巢刺激方案、卵母细胞成熟度、受精和囊胚质量相关。在FSH+LH方案中，17-羟基孕酮、雄烯二酮、雌二醇和雌酮显著升高。孕酮、17-羟基孕酮和雌二醇在产生成熟卵母细胞的FF中表达较多(p
In this prospective study, we evaluated the steroid levels in 111 follicular fluids (FF) collected from 13 women stimulated with FSH monotherapy and 205 FF collected from 28 women stimulated with FSH+LH because of a previous history of hypo-responsiveness to FSH. Steroid levels were measured by HPLC/MS-MS and related to ovarian stimulation protocol, oocyte maturity, fertilization and quality of blastocysts, after individually tracking the fate of all retrieved oocytes. 17-Hydroxy-Progesterone, Androstenedione, Estradiol and Estrone were significantly higher in the FSH+LH protocol. Progesterone, 17-Hydroxy-Progesterone and Estradiol were more expressed in FF yielding a mature oocyte (p