NG2-expressing cells in the subventricular zone are type C-like cells and contribute to interneuron generation in the postnatal hippocampus.
NG2-expressing cells in the subventricular zone are type C-like cells and contribute to interneuron generation in the postnatal hippocampus.
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DOI：
10.1083/jcb.200311141
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发表时间：
2004-05-24
影响因子：
7.8
通讯作者：
Gallo, Vittorio
中科院分区：
文献类型：
--
作者：
Aguirre, Adan A;Chittajallu, Ramesh;Belachew, Shibeshih;Gallo, Vittorio
脑室下区（SVZ）在整个大脑发育过程中是神经祖细胞的一个来源。对这些祖细胞的识别和纯化以及对其谱系潜能的分析是未来大脑修复疗法的基本问题。我们证明，位于脑室下区的出生后早期表达NG2（NG2 +）的祖细胞在体外能自我更新，并表现出类似C型转运放大多能细胞的表型特征。脑室下区的NG2 +细胞具有高度增殖能力，并表达表皮生长因子受体、转录因子Dlx、Mash1和Olig2以及Lewis X（LeX）抗原。我们表明，移植的出生后早期NG2 +细胞可产生海马的γ-氨基丁酸（GABA）能中间神经元，这些中间神经元能传播动作电位并接收功能性的谷氨酸能突触输入。我们的工作将脑室下区的Dlx + /Mash1 + /LeX + /NG2 + /胶质纤维酸性蛋白（GFAP）阴性细胞确定为一类新的出生后多能祖细胞，它可能代表了海马中出生后及成年抑制性中间神经元更新的一种特定细胞储备。
The subventricular zone (SVZ) is a source of neural progenitors throughout brain development. The identification and purification of these progenitors and the analysis of their lineage potential are fundamental issues for future brain repair therapies. We demonstrate that early postnatal NG2-expressing (NG2+) progenitor cells located in the SVZ self-renew in vitro and display phenotypic features of transit-amplifier type C–like multipotent cells. NG2+ cells in the SVZ are highly proliferative and express the epidermal growth factor receptor, the transcription factors Dlx, Mash1, and Olig2, and the Lewis X (LeX) antigen. We show that grafted early postnatal NG2+ cells generate hippocampal GABAergic interneurons that propagate action potentials and receive functional glutamatergic synaptic inputs. Our work identifies Dlx+/Mash1+/LeX+/NG2+/GFAP-negative cells of the SVZ as a new class of postnatal multipotent progenitor cells that may represent a specific cellular reservoir for renewal of postnatal and adult inhibitory interneurons in the hippocampus.