Differential effect of cis-eicosenoic acid positional isomers on adipogenesis and lipogenesis in 3T3-L1 cells
Differential effect of cis-eicosenoic acid positional isomers on adipogenesis and lipogenesis in 3T3-L1 cells
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顺式二十碳烯酸位置异构体对 3T3-L1 细胞脂肪生成和脂肪生成的差异作用
DOI：
10.1002/ejlt.201700512
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
and N. Gotoh
中科院分区：
文献类型：
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作者：
S. Senarath;K. Yoshinaga;T. Nagai;A. Yoshida;F. Beppu;and N. Gotoh
富含顺式二十碳烯酸的鱼油对肥胖相关的代谢功能障碍具有有益的健康作用。使用3 T3-L1前脂肪细胞检查了6种生物可利用的顺式二十碳烯酸位置异构体（c-20：1 PI）对脂肪生成和脂质蓄积的影响。使用实验（添加c-20：1 PI，50 μM）或对照（油酸（c9 - 18：1，50 μM）或无脂肪酸）培养基培养细胞。与c 9 - 18：1相比，c-20：1 PI治疗显著（p< 0.05）降低了细胞甘油三酯含量。细胞以显著（p< 0.05）不同的百分比与20：1 PI结合，并改变了脂肪酸代谢。Thec 15 - 20：1相对下调了脂肪生成（过氧化物酶体增殖激活受体γ和CCCAT增强子结合蛋白α）和脂肪生成（固醇调节元件结合蛋白-1）的转录因子;因此，显示出相当的抗脂肪生成和抗脂肪生成作用。综上所述，c-20：1 PI对3 T3-L1细胞的脂肪生成和脂质积累的影响不同，双键的位置影响其功能。实际应用：通过改善膳食脂肪的组成来控制脂肪细胞中的脂肪生成和脂质积累，作为预防肥胖和后续疾病的措施是有用的。本研究证明了顺式二十碳烯酸（c-20：1）位置异构体（特别是c 15 - 20：1）在控制脂肪细胞中的脂肪生成和随后的脂质蓄积方面的有益作用。因此，富含20：1位置异构体的食物，如鱼油、植物种子和坚果，可以作为预防肥胖的功能性食物，也可以作为有效的减肥补充剂。使用3 T3-L1前脂肪细胞研究顺式二十碳烯酸位置异构体（c-20：1 PI）对脂肪生成和脂质积累的影响。使用实验（添加c-20：1 PI，50 μM）或对照（油酸（c9 - 18：1，50 μM）或无脂肪酸）培养基培养细胞。与c9 - 18：1相比，c20：1 PI，特别是c15 - 20：1的处理显著（p< 0.05）降低了细胞甘油三酯含量，随后减少了细胞中的脂质积累。
Cis‐eicosenoic acid‐rich fish oil have shown beneficial health effects toward obesity‐related metabolic dysfunctions. Six bioavailablecis‐eicosenoic acid positional isomers (c‐20:1 PIs) are examined for their effects on adipogenesis and lipid accumulation using 3T3‐L1 preadipocytes. Cells are cultured using experimental (c‐20:1 PIs‐added, 50 µM) or control (oleic acid (c9‐18:1, 50 µM) or no fatty acids) medium. The treatment ofc‐20:1 PIs, significantly (p< 0.05) decreases the cellular triglyceride content compared to that ofc9‐18:1. Cells absorbedc‐20:1 PIs at significantly (p< 0.05) different percentages and has altered the fatty acid metabolism. Thec15‐20:1 comparatively down‐regulated the transcriptional factors for adipogenesis (peroxisome proliferative‐activated receptor gamma and CCCAT enhancer binding protein alpha) and lipogenesis (sterol regulatory element binding protein‐1); thus, show comparative anti‐adipogenic and anti‐lipogenic effect. In conclusion,c‐20:1 PIs affect differently on adipogenesis and lipid accumulation in 3T3‐L1 cells and the position of the double bond inc‐20:1 influences on their functionality.Practical applications: The control of the adipogenesis and lipid accumulation in adipocytes via improving the composition of dietary fat is useful as a prevention measure for obesity and subsequent diseases. This study demonstrates the beneficial effect ofcis‐eicosenoic acid (c‐20:1) positional isomers particularlyc15‐20:1 in controlling the adipogenesis and subsequent lipid accumulation in adipocytes. Thus, food rich in 20:1 positional isomers, such as fish oil, plant seeds, and nuts are useful as functional foods for the prevention of obesity and could also use as effective weight loss supplements.The effect ofcis‐eicosenoic acid positional isomers (c‐20:1 PIs) on the adipogenesis and lipid accumulation is studied using 3T3‐L1 preadipocytes. Cells are cultured using experimental (c‐20:1 PIs‐added, 50 μM) or control (oleic acid (c9‐18:1, 50 μM) or no fatty acids) medium. The treatment ofc‐20:1 PIs, particularlyc15‐20:1 significantly (p< 0.05) decreases the cellular triglyceride content compared to that ofc9‐18:1 and subsequently reduces the lipid accumulation in cells.