Rat rotator cuff muscle responds differently from hindlimb muscle to a combined tendon-nerve injury
Rat rotator cuff muscle responds differently from hindlimb muscle to a combined tendon-nerve injury
复制标题
DOI：
10.1002/jor.22864
复制
发表时间：
2015-07-01
影响因子：
2.8
通讯作者：
Feeley, Brian T.
中科院分区：
文献类型：
--
作者：
Davies, Michael R.;Ravishankar, Bharat;Feeley, Brian T.
肩袖撕裂（RCT）是骨科医生最常见的肌肉骨骼损伤之一。临床上，肩袖大面积撕裂导致肩袖肌独特的病理生理变化，包括萎缩和大量脂肪浸润，这在其他骨骼肌中很少见。在啮齿动物模型中进行的RCT研究表明，这些组织学发现伴随着肌腱-神经联合损伤后Akt/哺乳动物雷帕霉素靶蛋白（mTOR）和转化生长因子（TGF-）通路的激活。本研究的目的是比较肌腱-神经联合损伤后肩袖肌和腓肠肌-后肢大肌的组织学和分子特征。损伤后6周，与肩袖相比，大鼠腓肠肌没有表现出明显的脂肪浸润。同样，脂肪形成标志物SREBP-1和PPAR以及TGF-经典途径在肩袖中上调，但在腓肠肌中不上调。我们的研究表明，大鼠肩袖和后肢肌肉的反应显着不同的肌腱神经联合损伤。在临床上，这些发现突出了肩袖对损伤的独特反应，并可能开始解释与其他肌肉肌腱损伤相比，大规模随机对照试验的不良结果。(c)2015骨科研究学会。由威利期刊公司出版J Orthop Res 33：1046-1053，2015.
Rotator cuff tears (RCTs) are among the most common musculoskeletal injuries seen by orthopaedic surgeons. Clinically, massive cuff tears lead to unique pathophysiological changes in rotator cuff muscle, including atrophy, and massive fatty infiltration, which are rarely seen in other skeletal muscles. Studies in a rodent model for RCT have demonstrated that these histologic findings are accompanied by activation of the Akt/mammalian target of rapamycin (mTOR) and transforming growth factor- (TGF-) pathways following combined tendon-nerve injury. The purpose of this study was to compare the histologic and molecular features of rotator cuff muscle and gastrocnemius musclea major hindlimb muscle, following combined tendon-nerve injury. Six weeks after injury, the rat gastrocnemius did not exhibit notable fatty infiltration compared to the rotator cuff. Likewise, the adipogenic markers SREBP-1 and PPAR as well as the TGF- canonical pathway were upregulated in the rotator cuff, but not the gastrocnemius. Our study suggests that the rat rotator cuff and hindlimb muscles differ significantly in their response to a combined tendon-nerve injury. Clinically, these findings highlight the unique response of the rotator cuff to injury, and may begin to explain the poor outcomes of massive RCTs compared to other muscle-tendon injuries. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1046-1053, 2015.