Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes
Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes
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DOI：
10.1016/s0092-8674(02)01053-x
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发表时间：
2002-11-15
期刊：
影响因子：
64.5
通讯作者：
Endo, T
中科院分区：
文献类型：
--
作者：
Yamamoto, H;Esaki, M;Endo, T
基于易位中间体的位点特异性光交联结果，我们鉴定了 Tim50，它是酵母 TIM23 输入机制的一个组成部分，它介导含有前序列的蛋白质穿过线粒体内膜的易位。 Tim50 锚定于线粒体内膜，将 C 端结构域暴露于膜间隙。 Tim50 与 Tim23 的 N 端膜间空间结构域相互作用。 Tim50 的功能缺陷可以通过蛋白质的耗尽或添加抗 Tim50 抗体来阻止蛋白质跨内膜易位。 TOM 复合体中积累的易位中间体与 Tim50 交联。我们建议 Tim50 与 Tim23 合作，促进易位蛋白从 TOM 复合物转移到 TIM23 复合物。
Based on the results of site-specific photocrosslinking of translocation intermediates, we have identified Tim50, a component of the yeast TIM23 import machinery, which mediates translocation of presequence-containing proteins across the mitochondrial inner membrane. Tim50 is anchored to the inner mitochondrial membrane, exposing the C-terminal domain to the intermembrane space. Tim50 interacts with the N-terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti-Tim50 antibodies block the protein translocation across the inner membrane. A translocation intermediate accumulated at the TOM complex is crosslinked to Tim50. We suggest that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the TIM23 complex.