Pushing, pulling, and squeezing our way to understanding mechanotransduction.
Pushing, pulling, and squeezing our way to understanding mechanotransduction.
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推、拉和挤压是我们理解机械传导的方式。
DOI：
10.1016/j.ymeth.2015.08.019
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Nelson,CelesteM
中科院分区：
文献类型：
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作者：
Siedlik,MichaelJ;Varner,VictorD;Nelson,CelesteM
机械转导通常是在力引起的分子构象变化的背景下描述的，但分子尺度的机械刺激是在体内复杂的多细胞组织结构的背景下产生的。因此，我们重点介绍并回顾了研究跨多个长度尺度的力转导的实验方法。我们首先讨论探测单个分子对施加力的响应的技术。然后，我们在长度范围内向上移动，重点介绍旨在揭示细胞如何将机械刺激转化为生化活动的技术。最后，我们讨论确定这些刺激如何在多细胞结构中产生的方法。我们期望未来的工作将结合这些长度尺度的技术，以提供对力转导的更全面的理解。
Mechanotransduction is often described in the context of force-induced changes in molecular conformation, but molecular-scale mechanical stimuli arisein vivoin the context of complex, multicellular tissue structures. For this reason, we highlight and review experimental methods for investigating mechanotransduction across multiple length scales. We begin by discussing techniques that probe the response of individual molecules to applied force. We then move up in length scale to highlight techniques aimed at uncovering how cells transduce mechanical stimuli into biochemical activity. Finally, we discuss approaches for determining how these stimuli arise in multicellular structures. We expect that future work will combine techniques across these length scales to provide a more comprehensive understanding of mechanotransduction.