Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.
Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.
复制标题
有丝分裂后的中心脱离和成熟导致多倍体滋养细胞巨细胞的中心体扩增。
DOI：
10.1091/mbc.e22-05-0182
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发表时间：
2022-11-01
影响因子：
3.3
通讯作者：
Stearns, Tim
中科院分区：
文献类型：
--
作者：
Buss, Garrison;Stratton, Miranda B.;Milenkovic, Ljiljana;Stearns, Tim
在细胞周期中，DNA复制通常与中心粒复制相结合。胎盘的滋养层巨细胞(TGC)经历内周期，导致多倍体，但其中心粒状态尚不清楚。我们使用TGC分化的细胞培养模型来检测中心粒和中心体的数量和性质。在分化前，滋养层干细胞(TSCs)在复制前有两个中心粒，在复制后有四个中心粒。我们发现，随着分化的进行，平均核面积增加了大约8倍，但大多数TGC没有超过4个中心粒。然而，这些中心粒变得分离，获得中心体蛋白，并可以形成微管。此外，一些TGC经历了中心粒的进一步复制和分离，导致数量显著增加。活体成像显示，脱离和分离是中心粒自主的，可以异步发生。当存在中心粒放大时，按照一个中心粒产生一个原着丝粒的标准机制进行。Plk4抑制抑制TGCs分化过程中中心粒的形成，但不影响内周期的进展。综上所述，TGC内圈中的中心粒经历了脱离和向中心体的转化。这增加了中心体的数量，但与DNA复制相比，增加的程度有限。
DNA replication is normally coupled with centriole duplication in the cell cycle. Trophoblast giant cells (TGCs) of the placenta undergo endocycles resulting in polyploidy but their centriole state is not known. We used a cell culture model for TGC differentiation to examine centriole and centrosome number and properties. Before differentiation, trophoblast stem cells (TSCs) have either two centrioles before duplication or four centrioles after. We find that the average nuclear area increases approximately eight-fold over differentiation, but most TGCs do not have more than four centrioles. However, these centrioles become disengaged, acquire centrosome proteins, and can nucleate microtubules. In addition, some TGCs undergo further duplication and disengagement of centrioles, resulting in substantially higher numbers. Live imaging revealed that disengagement and separation are centriole autonomous and can occur asynchronously. Centriole amplification, when present, occurs by the standard mechanism of one centriole generating one procentriole. PLK4 inhibition blocks centriole formation in differentiating TGCs but does not affect endocycle progression. In summary, centrioles in TGC endocycles undergo disengagement and conversion to centrosomes. This increases centrosome number but to a limited extent compared with DNA reduplication.
影响因子：
7.8
作者：
BALCZON, R;BAO, LM;ZIMMER, WE;BROWN, K;ZINKOWSKI, RP;BRINKLEY, BR
通讯作者：
BRINKLEY, BR
影响因子：
3.7
作者：
Morimoto H;Ueno M;Tanabe H;Kono T;Ogawa H
通讯作者：
Ogawa H