Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii.
Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii.
复制标题
莱茵衣藻鞭毛相关表型的化学筛选。
DOI：
10.1007/978-1-4939-7874-8_17
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Avasthi,Prachee
中科院分区：
文献类型：
--
作者：
Jack,Brittany;Avasthi,Prachee
单细胞绿色藻类莱茵衣原体的鞭毛与脊椎动物细胞的纤毛几乎相同，为研究纤毛发生提供了极好的模型。纤毛和鞭毛是重要的细胞器，用于运动和感受细胞外环境。纤毛结构的缺失或纤毛功能障碍可能会产生灾难性的后果，从糖尿病和肥胖到多囊肾病和智力迟钝。小分子抑制剂文库可用于在组装、长度、运动性、爆燃和细胞毒性方面筛选鞭毛相关表型。这些表型可以通过直接显微镜观察和定制设计的测定进行评估。这些方法确定了纤毛生物学的基本调节因子以及纤毛病的潜在治疗干预。
Flagella of the unicellular green algaChlamydomonas reinhardtiiare nearly identical to cilia of vertebrate cells and provide an excellent model to study ciliogenesis. Cilia and flagella are important organelles used for motility and sensing the extracellular environment. Abnormalities in cilia structure or ciliary dysfunction can have devastating consequences ranging from diabetes and obesity to polycystic kidney disease and mental retardation. Small-molecule inhibitor libraries can be used to screen for flagellum-associated phenotypes in assembly, length, motility, deflagellation, and cellular toxicity. These phenotypes can be assessed from direct microscopic visualization and custom-designed assays. These methods identify fundamental regulators of ciliary biology as well as potential therapeutic interventions for ciliopathies.