HUMAN DNA-SEQUENCES HOMOLOGOUS TO A PROTEIN CODING REGION CONSERVED BETWEEN HOMEOTIC GENES OF DROSOPHILA
HUMAN DNA-SEQUENCES HOMOLOGOUS TO A PROTEIN CODING REGION CONSERVED BETWEEN HOMEOTIC GENES OF DROSOPHILA
复制标题
DOI：
10.1016/0092-8674(84)90261-7
复制
发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
TJIAN, R
中科院分区：
文献类型：
--
作者：
LEVINE, M;RUBIN, GM;TJIAN, R
几个人的DNA序列是凭借同源性的高度保守的区域，已被确定在一些同源异型基因在果蝇中分离。对人类DNA的结构分析表明，在人类基因组中，与果蝇同源盒序列共享高度同源性的2个独立且不同的区域仅被5个内切酶分开。这些区域的序列测定表明，这两个人类DNA序列都包含一个能够编码61个氨基酸的区域，该区域与果蝇同源基因Antennapedia、fushi tarazu和Ultrabithorax的homoeo box结构域指定的肽序列具有> 90%的同源性。相比之下，位于190个核苷酸同源盒区域之外的人DNA序列与其他人克隆的侧翼序列或与已知果蝇同源异型基因的侧翼区域几乎没有序列同源性。
Several human DNA sequences were isolated by virtue of homology to a highly conserved region that has been identified in a number of homoeotic genes in Drosophila. Structural analysis of the human DNA indicate that 2 separate and distinct regions sharing a high degree of homology with the homeo box sequences of Drosophila are separated by only 5 kilobase in the human genome. Sequence determination of these regions reveals that both human DNA sequences contain a region capable of coding 61 amino acids, which shares > 90% homology with the peptide sequences specified by the homoeo box domain of Drosophila homoeotic genes, Antennapedia, fushi tarazu and Ultrabithorax. By contrast, the human DNA sequences lying outside of the 190 nucleotides homoeo box region share virtually no sequence homology, either with the flanking sequences of the other human clones or with flanking regions of the known Drosophila homoeotic genes.