Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury
Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury
复制标题
DOI：
10.1089/neu.2018.5793
复制
发表时间：
2019-02-01
影响因子：
4.2
通讯作者：
Honmou, Osamu
中科院分区：
文献类型：
--
作者：
Oshigiri, Tsutomu;Sasaki, Toru;Honmou, Osamu
最近的证据表明，脊髓损伤（SCI）后，大脑中的远程反应，以及在受伤的脊髓局部反应，可以诱导。静脉输注间充质干细胞（MSC）已被证明可以通过局部治疗机制改善SCI的功能，这些机制提供神经保护，稳定血脊髓屏障，髓鞘再生和轴突发芽。在本研究中，我们研究了脑反应，这可能与脊髓损伤后注入MSC诱导的功能改善有关。在MSC或载体输注后3天，在SCI大鼠的运动皮层中进行全基因组RNA分析。定量逆转录-聚合酶链反应（qRT-PCR）结果显示，行为相关的差异表达基因（DEG）与行为功能相关，提示行为相关的差异表达基因可能与脊髓损伤后骨髓间充质干细胞系统输注后的功能恢复有关。这些结果表明，输注的MSC改变了大脑中的基因表达特征，这些表达变化可能有助于改善SCI的功能。
Recent evidence has demonstrated that remote responses in the brain, as well as local responses in the injured spinal cord, can be induced after spinal cord injury (SCI). Intravenous infusion of mesenchymal stem cells (MSCs) has been shown to provide functional improvements in SCI through local therapeutic mechanisms that provide neuroprotection, stabilization of the blood-spinal cord barrier, remyelination, and axonal sprouting. In the present study, we examined the brain response that might be associated with the functional improvements induced by the infused MSCs after SCI. Genome-wide RNA profiling was performed in the motor cortex of SCI rats at 3 days post-MSC or vehicle infusion. Then, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) data revealed that the behaviorally-associated differentially expressed genes (DEGs) were identified by the Pearson's correlation analysis with the behavioral function, suggesting that the behaviorally-associated DEGs may be related to the functional recovery after systemic infusion of MSCs in SCI. These results suggested that the infused MSCs alter the gene expression signature in the brain and that these expression changes may contribute to the improved function in SCI.