Integrative transcriptome, proteome, phosphoproteome and genetic mapping reveals new aspects in a fiberless mutant of cotton.
Integrative transcriptome, proteome, phosphoproteome and genetic mapping reveals new aspects in a fiberless mutant of cotton.
复制标题
整合转录组、蛋白质组、磷酸蛋白质组和遗传图谱揭示了棉花无纤维突变体的新方面
DOI：
10.1038/srep24485
复制
发表时间：
2016-04-14
影响因子：
4.6
通讯作者：
Yu SX
中科院分区：
文献类型：
--
作者：
Ma QF;Wu CH;Wu M;Pei WF;Li XL;Wang WK;Zhang J;Yu JW;Yu SX
为了研究棉花纤维发生的分子机制，采用转录组、基于iTRAQ的蛋白质组和遗传作图相结合的综合方法来比较徐州142野生型（WT）与其无绒毛无绒（fl）突变体在开花后-3天和0天的胚珠。结果表明，野生型与野生型之间共有1，953条mRNA、187个蛋白质和131个磷蛋白差异表达（DE），转录本及其编码的蛋白质和磷蛋白水平高度一致。功能分析表明，蛋白质主要参与氨基酸、核苷酸和脂肪酸的代谢，是叶酸代谢和类黄酮合成的碳库。进行qRT-PCR、蛋白质印迹和酶测定以确认这些转录物和蛋白质的调节。一个分子定位首次将无棉绒基因定位在26号染色体上。进一步的计算机物理作图的DE基因之间的序列变异的f1和WT确定了一个和四个候选基因，分别在113和n2区域。总之，转录本丰度，磷酸化状态的蛋白质在纤维起始阶段和候选基因提供了深入了解棉花纤维起始的调控过程。
To investigate the molecular mechanisms of fiber initiation in cotton (Gossypiumspp.), an integrated approach combining transcriptome, iTRAQ-based proteome and genetic mapping was taken to compare the ovules of the Xuzhou 142 wild type (WT) with its fuzzless-lintless (fl) mutant at −3 and 0 day post-anthesis. A total of 1,953 mRNAs, 187 proteins and 131 phosphoproteins were differentially expressed (DE) between WT andfland the levels of transcripts and their encoded proteins and phosphoproteins were highly congruent. A functional analysis suggested that the abundance of proteins were mainly involved in amino sugar, nucleotide sugar and fatty acid metabolism, one carbon pool for folate metabolism and flavonoid biosynthesis. qRT-PCR, Western blotting and enzymatic assays were performed to confirm the regulation of these transcripts and proteins. A molecular mapping located the lintless geneli3in theflmutant on chromosome 26 for the first time. A further in-silico physical mapping of DE genes with sequence variations betweenfland WT identified one and four candidate genes in theli3andn2regions, respectively. Taken together, the transcript abundance, phosphorylation status of proteins at the fiber initiation stage and candidate genes have provided insights into regulatory processes underlying cotton fiber initiation.