The effect of cold exposure on serum cholesterol is dependent upon ApoE
The effect of cold exposure on serum cholesterol is dependent upon ApoE
复制标题
寒冷暴露对血清胆固醇的影响取决于 ApoE
DOI：
10.1016/j.jtherbio.2021.102972
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发表时间：
2021-04-28
影响因子：
2.7
通讯作者：
Wu, Shengxian
中科院分区：
文献类型：
--
作者：
He, Fang;Su, Wenquan;Wu, Shengxian
背景资料：大量研究表明，冷刺激不仅可以激活棕色脂肪组织（BAT）和白色脂肪组织（WAT），还可以调节脂质代谢，影响动脉粥样硬化的发生发展。然而，冷暴露对胆固醇代谢影响的研究在不同的实验中得到了相反的结果，载脂蛋白E（ApoE）可能起着重要的作用。目前还缺乏完整的研究来说明这个问题。研究方法：在这项研究中，我们首先分析和讨论了高脂饮食（HFD）喂养4周的雄性野生型C57 BL/6 J（WT）和ApoE-/-小鼠（ApoE-/-）在冷暴露（4 ℃）下肩胛间棕色脂肪组织（iBAT）、腹股沟白色脂肪组织（iWAT）和附睾白色脂肪组织（eWAT）的活化。随后，我们研究了冷暴露对两种模型血脂谱的影响。我们进一步探讨了冷暴露是否可以降低血清胆固醇。结果：在WT和ApoE- /-小鼠中，冷暴露激活iBAT和iWAT，并且几乎不影响eWAT。在WT小鼠中，4周的冷暴露（4摄氏度）使血清甘油三酯降低28%，胆固醇降低30%，LDL胆固醇降低63%。在Apoe- /-小鼠中，冷刺激使血清甘油三酯降低59%，但胆固醇增加20%，低密度脂蛋白胆固醇增加25%。结论：基于这些发现，我们得出结论，冷暴露降低血清胆固醇是依赖于ApoE的存在。
Background: Several lines of evidence indicate that cold stimulation may not only activate brown adipose tissue (BAT) and the white adipose tissue (WAT), but also regulate the lipid metabolism and influence the development of atherosclerosis. However, the study of cold exposure affecting cholesterol metabolism have opposite results in different experiments, and Apolipoprotein E (ApoE) may play an important role. There is still a lack of complete research to illustrate this problem. Methods: In this study, we first analyzed and discussed the activation of interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) under cold exposure (4 degrees C) in male wild-type C57BL/6 J (WT) and ApoE-deficient mice (ApoE-/- ) fed high-fat diet (HFD) for 4 weeks. Subsequently, we investigated the effect of cold exposure on blood lipid profiles in both models. We further explored whether cold exposure can reduce serum cholesterol. Results: In both WT and ApoE- /- mice, cold exposure activates iBAT and iWAT, as well as hardly affects eWAT. In WT mice,4 weeks cold exposure (4 degrees C) reduces serum triglyceride by 28%, cholesterol by 30% and LDLcholesterol by 63%. In ApoE- /- mice, cold stimulation decreases serum triglyceride by 59%, but increases cholesterol by 20% and LDL-cholesterol by 25%. Conclusions: Based on these findings, we conclude that cold exposure decreases serum cholesterol is dependent upon the existence of ApoE.