N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
复制标题
DOI：
10.1179/1351000213y.0000000066
复制
发表时间：
2013-11-01
期刊：
影响因子：
3.8
通讯作者：
Guerra, L. N.
中科院分区：
文献类型：
--
作者：
Calzadilla, P.;Gomez-Serrano, M.;Guerra, L. N.
目的：氧化应激在糖尿病、高血压和动脉粥样硬化的发病机制中起着关键作用，但其对脂肪蓄积的影响尚不清楚。在这项研究中，我们分析了众所周知的抗氧化剂和谷胱甘肽（GSH）前体N-乙酰半胱氨酸（NAC）在脂肪积累和肥胖相关蛋白表达中的作用。方法：我们研究了10 μ M NAC对培养的3 T3-L1前脂肪细胞中肥胖相关蛋白表达的影响，这些细胞能够分化为成熟脂肪细胞并积累脂质。NAC治疗抑制脂肪的积累，减少肥胖相关蛋白的表达，包括单胺氧化酶A，热休克蛋白70（HSP 70），氨基酰化酶-1（ACY-1），和transketolase.Discussion：我们的研究结果表明，NAC对甘油三酯（TGS）和蛋白质表达的影响是相关的。为了支持这一点，我们表明NAC治疗影响了Tg合成途径和与人类肥胖有关的蛋白质的表达水平。
Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins.Methods: We studied the effects of 10 mu M NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids.Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase.Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.