ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF THE NEWBORN INFANT.
ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF THE NEWBORN INFANT.
复制标题
新生儿血浆中游离氨基酸的离子交换色谱。
作者：
J. Dickinson;H. Rosenblum;P. Hamilton
1.本文应用离子交换色谱微量测定氨基酸的方法，对0.1ml新生儿血浆中游离氨基酸进行了定量测定。该方法的精密度一般在± 5%范围内。给出了一个典型色谱图的解释分析。
2.血浆最好在-68 ° C下储存以待分析;指出在-20 ° C和更高温度下储存可能会出现错误。指出了无蛋白血浆的制备和贮存中可能出现的误差。
3.本文对25例新生儿股静脉血浆游离氨基酸与成人血浆游离氨基酸的文献值进行了比较。注意到的差异并不大，但显然是特征性的。比较足跟穿刺血浆，一些氨基酸高于股静脉血浆，但可以得出结论，足跟穿刺血浆将是令人满意的常规临床目的。注意到可能解释足跟穿刺血浆中氨基酸升高的一些可能的误差来源。
4.新生儿与3日龄婴儿的比较，注意到氨基酸水平的一些变化。变化不大，但似乎很有特点，而且前后一致。
5.色谱图上显示了许多之前在血浆中未观察到的小峰。未对它们进行鉴别，但提及它们是为了提请注意血浆中迄今未识别的茚三酮阳性成分的可能性。
1. A micromethod for the determination of amino acids by ion exchange chromatography was applied to the quantitative determination of the free amino acids in 0.1 ml of plasma of the newborn infant. The precision of the method was in general within the range ± 5%. An interpretive analysis of a typical chromatogram is given.
2. Plasma was best stored at -68°C pending analysis; errors likely to arise on storage at -20°C and higher temperatures are pointed out. Some errors likely to arise in the preparation and storage of plasma protein free filtrates are also indicated.
3. Comparison of the free amino acids in the femoral vein plasma of 25 newborn infants was made with the literature values of the free amino acids in the plasma of adults. The differences noted were not great, but were apparently characteristic. On comparing heel puncture plasma, some amino acids were higher than in femoral vein plasma, but it was concluded that heel puncture plasma would be satisfactory for routine clinical purposes. Some possible sources of error which might explain the elevated amino acids in the heel puncture plasma were noted.
4. On comparison of newborn with 3-day-old infants, some changes in amino acid levels were noted. The changes were not great, but seemed to be characteristic and consistent.
5. A number of small peaks which have not been noted previously in plasma have been indicated on the chromatogram. They were not identified, but were mentioned to draw attention to the possibilities of hitherto unrecognized ninhydrin-positive components of plasma.