Expression of serum albumin and of alphafetoprotein in murine normal and neoplastic primitive embryonic structures.
Expression of serum albumin and of alphafetoprotein in murine normal and neoplastic primitive embryonic structures.
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小鼠正常和肿瘤原始胚胎结构中血清白蛋白和甲胎蛋白的表达。
DOI：
10.1002/mrd.1080420402
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ilan,J
中科院分区：
文献类型：
--
作者：
Trojan,J;Naval,X;Johnson,T;Lafarge-Frayssinet,C;Hajeri-Germond,M;Farges,O;Pan,Y;Uriel,J;Abramasky,O;Ilan,J
甲胎蛋白（AFP）是一种主要的血清蛋白，在胚胎-胎儿和出生后合成（在卵黄囊中，然后在肝脏中），也是一种癌蛋白。细胞内AFP和血清白蛋白（SA）存在于正常和肿瘤神经嵴和神经管衍生物中。在这项工作中，我们研究了AFP和SA在小鼠胚胎（性交后6天和7天）和小鼠畸胎瘤（来自PCC4细胞系）的原始神经外胚层结构中的比较表达。利用免疫荧光技术，在7天的胚胎中检测到SA抗体阳性，而在此期间未检测到AFP。通过mRNA原位杂交，SA mRNA在6天和7天的胚胎中都能发出强信号，而AFP mRNA仅在7天的胚胎中发出弱信号。采用原位杂交和免疫染色的方法研究了SA和AFP及其mrna在畸胎瘤原始神经外胚细胞结构中的分布。免疫染色仅检测到SA蛋白。SA mRNA在分化结构和未分化的细胞簇中都有很强的信号。AFP mRNA仅在分化结构中可见。斑点杂交表明，在研究的畸胎癌中，SA转录本的水平至少比AFP转录本高6倍。在携带畸胎癌的小鼠腹腔注射125i放射性标记的SA和AFP后，SA和AFP在肿瘤中都有明显的积累，在肝脏摄取量正常化后，SA比AFP高约3倍。外部体内光扫描证实了这种积累的放射性标记蛋白的关系。最后的观察结果可用于体内畸胎瘤的诊断。我们得出结论，在正常胚胎发育组织和畸胎癌相应形态的肿瘤组织中，相对于AFP的SA表达和相对于AFP的SA的外部细胞摄取是相似的。同样的SA和AFP关系构成了原始神经外胚结构的癌胎标记。©1995 wiley‐Liss, Inc。
Alphafetoprotein (AFP), a major serum protein synthesized during the embryo‐fetal and postnatal period (in the yolk sac, then in the liver), is also an oncoprotein. The intracellular presence of AFP and of serum albumin (SA) in normal and neoplastic neural crest and neural tube derivatives was previously demonstrated. In this work we have studied the comparative expression of AFP and SA in primitive neuroectoblastic structures of mouse embryos (6 and 7 days “post coitum”) and mouse teratocarcinomas (derived from the PCC4 cell line). Using immunofluorescence technique, antibodies to SA gave a positive reaction in embryos of 7 days, while AFP was not detected during this period. By mRNA in situ hybridization, SA mRNA gave a strong signal in both 6 and 7 day embryos, whereas AFP mRNA gave a weak signal only in 7‐day embryos. The distribution of SA and AFP and their mRNAs was investigated in primitive neuroectoblastic structures of the teratocarcinomas by in situ hybridization and immunostaining. Only SA protein was detectable by immunostaining. SA mRNA gave a strong signal in differentiating structures as well as in undifferentiated cell clusters. AFP mRNA was observed only in differentiating structures. Dot‐blot hybridization indicated that the level of SA transcripts was at least 6‐fold higher than that of AFP transcripts in the teratocarcinomas investigated. In teratocarcinoma‐bearing mice injected intraperitoneally with125I‐radiolabeled SA and AFP, significant accumulations of both SA and AFP were demonstrated in the tumors, SA being about 3‐fold higher than that of AFP after normalization to quantity of uptake in liver. External in vivo photoscanning confirmed this relationship of accumulated radiolabeled proteins. The last observation could be useful in vivo for diagnosis of teratocarcinoma. We conclude that the expression of SA relative to AFP and the external cellular uptake of SA relative to AFP are similar in normal embryonic developing tissues and in the corresponding morphologically neoplastic tissues of the teratocarcinomas. The same SA:AFP relationship constitutes an oncofetal marker of primitive neuroectoblastic structures. © 1995 wiley‐Liss, Inc.