Reversible DNA micro-patterning using the fluorous effect.

Reversible DNA micro-patterning using the fluorous effect.
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DOI:
10.1039/c7cc00288b
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发表时间:
2017-03-09
期刊:
Chemical communications (Cambridge, England)
影响因子:
--
通讯作者:
Clark AW
Clark AW
中科院分区:
其他
文献类型:
--
作者:
Flynn GE;Withers JM;Macias G;Sperling JR;Henry SL;Cooper JM;Burley GA;Clark AW

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我们将荧光效应的使用描述为一种简单且可逆的 DNA 固定技术。我们描述了一种利用荧光效应将 DNA 固定到亚微米分辨率的确定图案的新方法。该方法通过简单的溶剂清洗是完全可逆的,允许表面图案化、再生和重新图案化,并且在不同DNA序列的多次去除/附着循环后,结合效率不会降低。
We described the use of the fluorous effect as a simple and reversible immobilisation technique for DNA. We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.