INTRA-CYTOPLASMIC MEMBRANE, PHOSPHOLIPID, AND STEROL CONTENT OF METHYLOBACTERIUM-ORGANOPHILUM CELLS GROWN UNDER DIFFERENT CONDITIONS
INTRA-CYTOPLASMIC MEMBRANE, PHOSPHOLIPID, AND STEROL CONTENT OF METHYLOBACTERIUM-ORGANOPHILUM CELLS GROWN UNDER DIFFERENT CONDITIONS
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DOI：
10.1128/jb.134.2.636-644.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
HANSON, RS
中科院分区：
文献类型：
--
作者：
PATT, TE;HANSON, RS
M.当细胞用甲烷而不是甲醇或葡萄糖作为唯一的C和能量来源生长时，以甲烷为碳源和能源，低水平溶解氧生长的细胞具有最大量的胞质膜。随着溶解O2水平的增加而生长的细胞具有较少的胞质膜。总脂质的量与在薄切片中观察到的膜材料的量相关。单个磷脂的含量不同，但M中存在相同的4种磷脂。在不同的基质和O2水平下生长。磷脂酰胆碱作为磷脂的主要成分存在。存在硬脂酸，它们不同于那些在I型甲基营养菌荚膜甲基球菌。不同甾醇和角鲨烯的相对量随提供用于生长的底物而变化。最大量的甾醇被发现在甲烷生长的细胞生长在低水平的溶解O2。非异常或常见的膜成分分析是唯一存在于胞质膜。
Intracytoplasmic membranes were present in M. organophilum when cells were grown with methane, but not methanol or glucose, as the sole C and energy source. Cells grown with methane as the C and energy source and low levels of dissolved O2 had the greatest amount of intracytoplasmic membrane. Cells grown with increased levels of dissolved O2 had less intracytoplasmic membrane. The amount of total lipid correlated with the amount of membrane material observed in thin sections. The individual phospholipids varied in amount, but the same 4 were present in M. organophilum grown with different substrates and O2 levels. Phosphatidyl choline was present as a major component of the phospholipids. Sterols were present and they differed from those in the type I methylotroph Methylococcus capsulatus. The relative amounts of different sterols and squalene changed with the substrate provided for growth. The greatest amounts of sterols were found in methane-grown cells grown at low levels of dissolved O2. Non of the unusual or usual membrane components assayed was uniquely present in the intracytoplasmic membranes.