Reconstitution of HLA-A*2402-restricted cytomegalovirus-specific T-cells following stem cell transplantation
Reconstitution of HLA-A*2402-restricted cytomegalovirus-specific T-cells following stem cell transplantation
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DOI：
10.1532/ijh97.04109
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发表时间：
2004-12-01
影响因子：
2.1
通讯作者：
Harada, M
中科院分区：
文献类型：
--
作者：
Gondo, H;Himeji, D;Harada, M
通过检测CD8+T细胞识别HLAA*2402中的QYDPVAALF多肽，前瞻性地评价干细胞移植(SCT)后早期巨细胞病毒(CMV)特异性免疫重建。15名同种异体SCT受者参与了这项研究。所有受者和供者均为CMV血清阳性，且均携带人类白细胞抗原-A*2402等位基因。移植后1个月即可检测到CMV特异性T细胞，移植后2~5个月达到高峰。发生II~IV级急性移植物抗宿主病(GVHD)并接受糖皮质激素治疗的急性移植物抗宿主病(GVHD)患者在SCT后早期CMV特异性T细胞数量较低。异基因外周血干细胞移植(PBSCT)患者较异基因骨髓移植患者有更早重建CMV特异性CD8+T细胞的趋势。CD34选择的自体PBSCT与未净化的自体PBSCT相比，CMV特异性CD8+T细胞的重建延迟了移植物中T细胞在CMV特异性免疫反应恢复中的作用。在CMV病或CMV复发患者中，CMV特异性CD8+T细胞的重建延迟。这些观察表明，用H-LA-肽四聚体检测CMV特异性T细胞有助于评估针对CMV的免疫重建，并识别SCT后CMV病或复发CMV重新激活的风险。(C)2004年日本血液病学会
Cytomegalovirus (CMV)-specific immune reconstitution early after stem cell transplantation (SCT) was evaluated prospectively by detecting CD8+ T-cells, which recognize the peptide QYDPVAALF in the context of HLA-A*2402. Fifteen allogeneic SCT recipients were included in the study. All recipients and donors were seropositive for CMV and had the HLA-A*2402 allele. CMV-specific T-cells were detected as early as 1 month after transplantation, and their numbers increased to peak levels 2 to 5 months after transplantation. The numbers of CMV-specific T-cells in patients who developed grade II to IV acute graft-versus-host disease (GVHD) and received corticosteroids for acute GVHD were low in the early period after allogeneic SCT. There was a trend toward earlier reconstitution of CMV-specific CD8+ T-cells in allogeneic peripheral blood SCT (PBSCT) patients than in allogeneic bone marrow transplantation patients. The contribution of T-cells in the graft to the recovery of CMV-specific immune responses was also suggested by the finding that the reconstitution of CMV-specific CD8+ T-cells was delayed in CD34-selected autologous PBSCT compared with unpurged autologous PBSCT. The reconstitution of CMV-specific CD8+ T-cells was delayed in patients with CMV disease or recurrent CMV reactivation. These observations suggest that the detection of CMV-specific T-cells with an H LA-peptide tetramer is useful to assess immune reconstitution against CMV and to identify patients at risk for CMV disease or recurrent CMV reactivation after SCT. (C) 2004 The Japanese Society of Hematology