Fascin1 expression predicts poor prognosis in patients with nasopharyngeal carcinoma and correlates with tumor invasion
Fascin1 expression predicts poor prognosis in patients with nasopharyngeal carcinoma and correlates with tumor invasion
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Fascin1 表达预测鼻咽癌患者预后不良，并与肿瘤侵袭相关。
DOI：
10.1093/annonc/mdp333
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发表时间：
2010-03-01
影响因子：
50.5
通讯作者：
Liu, L.
中科院分区：
文献类型：
--
作者：
Wu, D.;Chen, L.;Liu, L.
背景：本研究的目的是探讨SENCE1在鼻咽癌中的预后意义及其与肿瘤侵袭性的关系。材料和方法：对161例鼻咽癌石蜡包埋组织切片进行免疫组织化学染色。对临床病理变量和存活率的数据进行统计分析。采用小分子干扰RNA(SiRNA)技术，通过对鼻咽癌细胞中Zocor1基因的表达进行下调，以确定其在鼻咽癌细胞侵袭中的作用。结果：免疫组织化学分析显示，在161例石蜡包埋的鼻咽癌组织中，有95例(59.0%)呈高表达。Fascin1的表达与临床分期(P<；0.001)和N分期(P<；0.001)显著相关。统计分析表明，SENCE1基因的表达与鼻咽癌患者的总体生存率和无瘤生存率呈负相关。多因素分析显示，SENCE1表达是影响患者生存的独立预后指标。此外，用siRNA技术阻断鼻咽癌细胞内源性ZOSE1的表达，可抑制鼻咽癌细胞的侵袭性，减少细胞的丝足和板足。结论：本研究表明，SENCE1的表达与鼻咽癌患者的生存期呈负相关，与鼻咽癌的恶性程度呈正相关。
Background: The purpose of this study was to investigate prognostic significance of fascin1 in nasopharyngeal carcinoma (NPC) and evaluate the association of fascin1 with tumor invasion.Materials and methods: Immunohistochemical staining for fascin1 was carried out on paraffin-embedded tissue sections from 161 patients with NPC. Data were subjected to statistical analysis with respect to clinicopathological variables and survival. Small interfering RNA (siRNA) approach was used to knockdown fascin1 expression in NPC cells to determine whether fascin1 contributes to tumor cell invasion.Results: Immunohistochemical analysis showed that fascin1 was highly expressed in 95 (59.0%) of 161 paraffin-embedded NPC tissues. Fascin1 expression was significantly correlated with clinical stage (P < 0.001) and N classification (P < 0.001). Statistical analysis showed that fascin1 expression was inversely correlated with both overall and disease-free survival of NPC patients. Multivariate analysis showed that fascin1 expression was an independent prognostic indicator for patient's survival. Moreover, disruption of endogenous fascin1 expression in NPC cells using siRNA technique suppressed NPC cell invasiveness and decreased cell filopodia and lamellopodia.Conclusion: The present study indicates that fascin1 expression is inversely correlated with NPC patient survival and directly correlated with the malignant status of NPC.