JNK and decapentaplegic signaling control adhesiveness and cytoskeleton dynamics during thorax closure in Drosophila
JNK and decapentaplegic signaling control adhesiveness and cytoskeleton dynamics during thorax closure in Drosophila
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DOI：
10.1073/pnas.97.14.7888
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发表时间：
2000-07-05
影响因子：
11.1
通讯作者：
García-Bellido, A
中科院分区：
文献类型：
--
作者：
Martín-Blanco, E;Pastor-Pareja, JC;García-Bellido, A
昆虫变态的基本事件之一是幼虫组织被成虫组织所取代。化蛹后不久，成虫盘外翻，在蛹前动物的表面占据它们的位置。这是一个非常精确的过程，只是刚刚开始被理解。在果蝇中，在胚胎背闭合期间，上皮细胞推动浆膜细胞，浆膜细胞收缩并最终内陷在体腔中。相反，我们发现在蛹化期间，成虫细胞遵循非常准确的时间和空间模式在被动的幼虫组织上爬行。伸展是由丝状伪足和肌动蛋白桥驱动的，从前缘突出，介导成虫上皮细胞的拉伸。虽然干扰JNK（Jun N-末端激酶）和DPP（decapentaplegic）产生类似的抑制闭合的表型效应，但它们在细胞水平上的效应是不同的。JNK活性的丧失改变了幼虫细胞的粘附特性，并导致成虫和幼虫组织的分离。dpp信号的缺失影响肌动蛋白细胞骨架，阻止丝状伪足的发射，并促进成虫组织前缘的塌陷。有趣的是，这些影响是非常相似的，在胚胎背闭合过程中干扰JNK和DPP信号转导后观察到的。
One of the fundamental events in metamorphosis in insects is the replacement of larval tissues by imaginal tissues. Shortly after pupariation the imaginal discs evaginate to assume their positions at the surface of the prepupal animal. This is a very precise process that is only beginning to be understood. In Drosophila, during embryonic dorsal closure, the epithelial cells push the amnioserosa cells, which contract and eventually invaginate in the body cavity, In contrast, we find that during pupariation the imaginal cells crawl over the passive larval tissue following a very accurate temporal and spatial pattern. Spreading is driven by filopodia and actin bridges that, protruding from the leading edge, mediate the stretching of the imaginal epithelia, Although interfering with JNK (Jun N-terminal kinase) and dpp (decapentaplegic) produces similar phenotypic effects suppressing closure, their effects at the cellular level are different. The loss of JNK activity alters the adhesion properties of larval cells and leads to the detachment of the imaginal and larval tissues. The absence of dpp signaling affects the actin cytoskeleton, blocks the emission of filopodia, and promotes the collapse of the leading edge of the imaginal tissues. Interestingly, these effects are very similar to those observed after interfering with JNK and dpp signaling during embryonic dorsal closure.