Expression and activity of the signaling molecules for mitogen-activated protein kinase pathways in human, bovine, and rat lenses.
Expression and activity of the signaling molecules for mitogen-activated protein kinase pathways in human, bovine, and rat lenses.
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人、牛和大鼠晶状体中丝裂原激活蛋白激酶途径信号分子的表达和活性。
DOI：
10.1167/iovs.03-0348
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发表时间：
2003
影响因子：
4.4
通讯作者：
Hou,Li-Hui
中科院分区：
文献类型：
--
作者：
Li,DavidWan-Cheng;Liu,Jin-Ping;Wang,Juan;Mao,Ying-Wei;Hou,Li-Hui
目的.丝裂原活化蛋白激酶（MAPK）通路在透镜中起着不同的作用。然而，这些途径的各种组分的表达模式和活性水平在脊椎动物晶状体，特别是人透镜中尚未得到充分证实。在本研究中，细胞外信号调节激酶（ERK）-1/2/3，c-Jun NH 2-末端激酶（JNK）-1/2，p38激酶，丝裂原活化蛋白激酶激酶（MEK）-1/2和RAF 1的表达和活性在人类，牛和大鼠晶状体中记录。从完整的眼睛中分离人、牛和大鼠晶状体。从这些晶状体中分离上皮细胞和不同层的纤维细胞。对从这些样品中提取的总蛋白进行MAPKs和ERK 1/2活化激酶的表达模式和活性水平的分析。ERK 1和ERK 2是所有晶状体中蛋白质和活性水平最丰富的MAPK。JNK 1和JNK 2在牛透镜中高度表达，这与人和大鼠晶状体中共有的模式不同。p38激酶在牛和大鼠晶状体中的表达相似，但与人透镜中的表达不同。然而，p38激酶活性仅在上皮细胞中检测到。所有晶状体上皮细胞均具有MEK 1/2活性，但这些晶状体中MEK 1和MEK 2的表达模式不同。RAF 1在所有晶状体上皮细胞中表达，但其活性仅在大鼠晶状体中检测到。ERK 1和ERK 2是眼透镜中含量最丰富的MAPK，为它们在透镜发育和发病中的多种功能提供了基础。JNK 1/2和p38激酶的优势上皮分布表明透镜上皮是应激反应的主要部位。ERK 1、p38激酶和PKCα可作为衰老的分子标志物。
purpose. The mitogen-activated protein kinase (MAPK) pathways play distinct roles in the lens. However, the expression patterns and activity levels of various components for these pathways have not been well-documented in vertebrate lenses, especially human lens. In the present study, the expressions and activities of extracellular signal-regulated kinase (ERK)-1/2/3, c-Jun NH2-terminal kinase (JNK)-1/2, p38 kinase, mitogen-activated protein kinase kinase (MEK)-1/2, and RAF1 were recorded in human, bovine, and rat lenses.methods. Human, bovine, and rat lenses were isolated from intact eyes. The epithelia and different layers of fiber cells were isolated from these lenses. Total proteins extracted from these samples were subject to analysis of the expression patterns and activity levels of the MAPKs and the activating kinases of ERK1/2.results. ERK1 and ERK2 were the most abundant MAPKs in terms of both protein and activity levels in all lenses. JNK1 and JNK2 were highly expressed in bovine lens, which differed from the pattern shared by human and rat lenses. p38 kinase was similarly expressed in bovine and rat lenses, but different from that in human lens. However, p38 kinase activity was exclusively detected in the epithelia. All lenses had MEK1/2 activity in their epithelia but the expression patterns of MEK1 and MEK2 differed in these lenses. RAF1 was expressed in the epithelia of all lenses, but its activity was detected only in rat lens.conclusions. ERK1 and ERK2 are the most abundant MAPKs in the ocular lens, providing the basis for their multiple functions in lens development and pathogenesis. The dominant epithelial distribution of JNK1/2 and p38 kinase suggests that the lens epithelium is a major site for stress response. ERK1, p38 kinase, and PKCα can be used as molecular markers for aging.