Shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia
Shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia
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DOI：
10.1101/gad.10.6.672
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发表时间：
1996-03-15
影响因子：
10.5
通讯作者：
Hartenstein, V
中科院分区：
文献类型：
--
作者：
Tepass, U;GruszynskiDeFeo, E;Hartenstein, V
钙粘附素超家族的黏附分子在脊椎动物的发育过程中起着重要作用。DE-钙粘蛋白同系物DE-钙粘蛋白是第一个从无脊椎动物中分离出来的经典钙粘蛋白。我们在这里报道了DE-钙粘蛋白是由猎枪(Shg)基因编码的。SHG在大多数胚胎上皮细胞中表达，在经历上皮-间充质转化的细胞中表达减少，如中胚层或神经前体细胞。母体和受精卵shg功能的去除会导致所有表达shg的上皮细胞的严重缺陷，这表明DE-cadherin类似于脊椎动物的经典钙粘附素，在上皮组织的形成和/或维持中起着至关重要的作用。有趣的是，对不同shg等位基因的分析表明，在给定的上皮中对shg的需求取决于其形态发生活动的程度。只有参与广泛形态发生运动的上皮细胞除了母体表达外，还需要合子shg功能。支持这一观点的是，我们发现抑制形态发生运动可以挽救合子shg表型。我们发现，在受精卵Shgnull中，Dα-连环素和Armadillo在贴壁连接处的水平显著降低，令人惊讶的是，在分化正常并具有贴壁带的上皮细胞中也是如此。
Adhesion molecules of the cadherin superfamily have an important role during vertebrate development. The DE-cadherin homolog DE-cadherin is the first classic cadherin isolated from invertebrates. We report here that DE-cadherin is encoded by the shotgun (shg) gene. shg is expressed in most embryonic epithelia and decreases in cells that undergo epithelial-mesenchymal transitions like the mesoderm or neural precursors. Removal of both maternal and zygotic shg function leads to severe defects in all epithelia expressing shg, suggesting that DE-cadherin, similar to vertebrate classic cadherins, has a crucial role for the formation and/or maintenance of epithelial tissues. Interestingly, the analysis of different shg alleles indicates that the requirement for shg in a given epithelium depends on the degree of its morphogenetic activity. Only epithelia involved in extensive morphogenetic movements require zygotic shg function in addition to maternal expression. In support of this view we find that suppression of morphogenetic movements rescues the zygotic shg phenotype. We find that in zygotic shg nulls the level of D alpha-catenin and Armadillo at adherens junctions is dramatically reduced, surprisingly also in epithelia that differentiate normally and possess a zonula adherens.