Lewis blood group fucolipids and their isomers from human and canine intestine.
Lewis blood group fucolipids and their isomers from human and canine intestine.
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来自人类和犬科动物肠道的路易斯血型岩藻糖脂及其异构体。
DOI：
10.1016/s0021-9258(19)68260-8
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
S. Li
中科院分区：
文献类型：
--
作者：
J. Mckibbin;W. A. Spencer;E. L. Smith;J. Månsson;K. Karlsson;B. Samuelsson;Y. Li;S. Li
从 14 个人和 13 个人狗肠道中分离并鉴定了含有与 N-乙酰氨基葡萄糖连接的岩藻糖的糖脂。从 8 个狗肠中分离出 Lewis a 异构体岩藻糖脂，全部相同，并具有结构 Gal(β 1 导致 4)[Fuc α 1 导致 3]GlcNAc(β 1 导致 3)Gal(β 1 导致 4)Glc-神经酰胺。从 12 个肠道中分离出 Lewis b 异构体岩藻糖脂，全部相同，并具有结构 Fuc(α 1 导致 2)Gal(β 1 导致 4)[Fuc α 1 导致 3]GlcNAc(β 1 导致 3)Gal(β 1 导致 4)Glc-神经酰胺。 Lewis a-活性糖脂是在 6 个人类肠道中分离出来的唯一主要岩藻糖脂，与犬类异构体的不同之处仅在于半乳糖与 N-乙酰氨基葡萄糖的连接位置，β 1 通向 3（1 型）而不是 β 1 通向 4（2 型）连接。 Lewis b 活性岩藻糖脂是从 8 个人类肠道中分离出来的，与犬类异构体的不同之处仅在于它们也具有 1 型而非 2 型寡糖链。 Lewis a 和 b 糖脂异构体通常作为主要岩藻糖脂共存于犬科动物肠道中，而 Lewis a 和 b 糖脂异构体在人肠道中则不然。在所有岩藻糖脂中，仅存在羟基化脂肪酸，并且植物鞘氨醇和鞘氨醇是主要的长链碱基。这些发现对于这些物质的生物合成及其基因表达很有意义。
Glycolipids containing fucose linked to N-acetylglucosamine were isolated and characterized from 14 individual human and 13 individual dog intestines. From 8 of the dog intestines, Lewis a isomer fucolipids were isolated, all identical and having the structure Gal(beta 1 leads to 4)[Fuc alpha 1 leads to 3]GlcNAc(beta 1 leads to 3)Gal(beta 1 leads to 4)Glc-ceramide. Lewis b isomer fucolipids were isolated from 12 of the intestines, all identical and having the structure Fuc(alpha 1 leads to 2)Gal(beta 1 leads to 4)[Fuc alpha 1 leads to 3]GlcNAc(beta 1 leads to 3)Gal(beta 1 leads to 4)Glc-ceramide. Lewis a-active glycolipids were isolated as the sole major fucolipid in 6 of the human intestines and differed from the canine isomer only in the position of the linkage of galactose to N-acetylglucosamine, having the beta 1 leads to 3 (type 1) rather than the beta 1 leads to 4 (type 2) linkage. Lewis b-active fucolipids were isolated from 8 human intestines and differed from their canine isomer only in that they, too, had the type 1 rather than the type 2 oligosaccharide chain. Lewis a and b glycolipid isomers commonly co-existed in canine intestine as major fucolipids whereas Lewis a and b glycolipids did not so co-exist in human intestine. In all of the fucolipids, only hydroxylated fatty acids were present and phytosphingosine and sphingosine were the predominant long chain bases. These findings are of interest in the biosynthesis of these substances and in their genetic expression.