Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana.
Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana.
复制标题
DOI：
10.1111/j.0960-7412.1997.00527.x
复制
发表时间：
1997-09
期刊：
影响因子：
--
通讯作者：
S. Oh;Joon-Hyun Park;Gyu In Lee;Kyung Hee Paek;Soon Ki Park;Hong Gil Nam
中科院分区：
文献类型：
--
作者：
S. Oh;Joon-Hyun Park;Gyu In Lee;Kyung Hee Paek;Soon Ki Park;Hong Gil Nam
从拟南芥中分离到4个表现延缓叶片衰老表型的突变体。遗传分析表明，它们都是单基因隐性突变，可分为3个互补组，确定了3个控制拟南芥叶片衰老的遗传位点。这些基因座的突变会导致所检测的所有衰老参数的延迟，包括叶绿素含量、光系统II的光化学效率、Rubisco大亚基的相对数量以及核糖核酸酶和过氧化物酶活性。无论是在植物衰老过程中，还是在暗诱导的人工衰老过程中，所有突变体都观察到衰老症状的延缓。结果表明，这三个基因是控制功能叶片衰老的关键遗传元件，为拟南芥叶片衰老是由多个单基因座位控制的遗传程序性现象提供了决定性的遗传证据。结果进一步表明，这三个基因在依赖年龄和黑暗诱导的衰老过程中起着共同的作用。进一步证明其中一个突变与乙烯不敏感突变ein2-1等位，证实了乙烯信号转导途径在拟南芥叶片衰老中的作用。
Four mutants that show the delayed leaf senescence phenotype were isolated from Arabidopsis thaliana. Genetic analyses revealed that they are all monogenic recessive mutations and fall into three complementation groups, identifying three genetic loci controlling leaf senescence in Arabidopsis. Mutations in these loci cause delay in all senescence parameters examined, including chlorophyll content, photochemical efficiency of photosystem II, relative amount of the large subunit of Rubisco, and RNase and peroxidase activity. Delay of the senescence symptoms was observed during both age-dependent in planta senescence and dark-induced artificial senescence in all of the mutant plants. The results indicate that the three genes defined by the mutations are key genetic elements controlling functional leaf senescence and provide decisive genetic evidence that leaf senescence is a genetically programmed phenomenon controlled by several monogenic loci in Arabidopsis. The results further suggest that the three genes function at a common step of age-dependent and dark-induced senescence processes. It is further shown that one of the mutations is allelic to ein2-1, an ethylene-insensitive mutation, confirming the role of ethylene signal transduction pathway in leaf senescence of Arabidopsis.