Early development of the myotome in the mouse
Early development of the myotome in the mouse
复制标题
小鼠肌节的早期发育
作者：
S. Venters;S. Thorsteinsdóttir;M. Duxson
肌节的结构和发育在鸟类和两栖动物中已被广泛研究，但很少有研究系统地解决其在哺乳动物中的发育。我们已经使用了一个转基因小鼠携带nLacZ标记耦合到肌球蛋白轻链3F启动子来描述发育中的哺乳动物肌节的结构。通过转基因表达模式的研究，再加上肌肉结构蛋白结蛋白和慢肌球蛋白重链的免疫组化，我们描述了一个梯度的成熟度为细胞内的发展肌节。我们的研究结果表明，最早的哺乳动物肌节的肌细胞跨越吻尾侧的体节的程度，并有单一的大核集中在肌节内。在整个研究期间，肌节腹侧比背侧更成熟，肌节内侧的细胞比外侧的细胞年轻。使用最早表达的肌肉调节因子（myf-5）的免疫组织化学来定义肌节中产生新肌源性细胞的区域。在早期肌节中，小而圆的myf-5表达细胞广泛存在于皮肌节的背内侧以及整个吻侧和尾侧皮肌节唇内。它们随后出现在肌节的中央区域，邻近内侧卷曲的吻侧和尾侧真皮肌节唇，并在那里开始对称地伸长。随着肌节的扩大，myf-5的表达总是局限于肌节的最内侧，与最不成熟的肌细胞相邻，标志着新的肌源性细胞的增加。总之，这些结果允许哺乳动物肌节形成模型的发展，其中通过从吻侧和尾侧dermamyotome唇添加新细胞而在内侧发生生长，而更成熟的肌细胞被横向移位。此外，早期肌瘤肌细胞分化的MyoD表达的情况下，不像后来的肌瘤肌细胞。这一点，沿着其独特的形态，表明这些细胞可能形成一个单独的谱系的先驱肌源性细胞。Dev Dyn 1999;216：219-232.©1999 Wiley利斯公司
The structure and development of the myotome has been extensively studied in birds and amphibians but few studies have systematically addressed its development in mammals. We have used a transgenic mouse carrying an nLacZ marker coupled to a myosin light chain 3F promoter to describe the structure of the developing mammalian myotome. Through studies of transgene expression pattern, coupled with immunohistochemistry for the muscle structural proteins desmin and slow myosin heavy chain we describe a gradient of maturity for the cells within the developing myotome. Our results show that the earliest myocytes of the mammalian myotome span the rostrocaudal extent of the somite and have single large nuclei which localise centrally within the myotome. Throughout the period of study the myotome is more mature ventrally than dorsally and cells comprising the medial aspect of the myotome are younger than those lying laterally. Immunohistochemistry for the earliest expressed muscle regulatory factor (myf‐5) is used to define areas of the myotome contributing new myogenic cells. In the early myotome small, round, myf‐5‐expressing cells are found extensively within the dorsomedial aspect of the dermamyotome and also within the entire rostral and caudal dermamyotomal lips. They subsequently appear within the central zone of the myotome, adjacent to the medially curled rostral and caudal dermamyotomal lips, and there begin to elongate symmetrically. As the myotome enlarges, myf‐5 expression is always restricted to the most medial aspect of the myotome, adjacent to the least mature myocytes, marking the site of addition of new myogenic cells. Together, these results allow development of a model of mammalian myotome formation where growth occurs medially by addition of new cells from both rostral and caudal dermamyotome lips, while more mature myocytes are displaced laterally. Furthermore, early myotomal myocytes differentiate in the absence of MyoD expression, unlike later myotomal myocytes. This, along with their distinct morphology, suggests these cells may form a separate lineage of pioneer myogenic cells. Dev Dyn 1999;216:219–232. ©1999 Wiley‐Liss, Inc.
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体节的生肌规范：轴向结构的诱导。
DOI：
10.1242/dev.120.6.1443
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Buffinger,N;Stockdale,FE
通讯作者：
Stockdale,FE
影响因子：
10.5
作者：
MUNSTERBERG, AE;KITAJEWSKI, J;LASSAR, AB
通讯作者：
LASSAR, AB
影响因子：
--
作者：
LIN, ZX;LU, MH;HOLTZER, H
通讯作者：
HOLTZER, H
外轴肌节前体细胞的位置和生长。
DOI：
10.1242/dev.124.8.1601
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
DenetclawJr,WF;Christ,B;Ordahl,CP
通讯作者：
Ordahl,CP
Pax-3 在节段中胚层中的表达标志着肌原细胞规范的早期阶段。
DOI：
10.1242/dev.120.4.785
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Williams,BA;Ordahl,CP
通讯作者：
Ordahl,CP